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AB172974

Anti-DNA Polymerase lambda antibody [EPR7519(2)]

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Rabbit Recombinant Monoclonal DNA Polymerase lambda/Polk antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
3 이미지
Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)
  • WB

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Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)

Lanes 1-3 : Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

POLL knockout HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)
  • WB

Lab

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)

Lanes 1-3 : Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

POLL knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human POLL (DNA Polymerase lambda) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-poll-dna-polymerase-lambda-knockout-hela-cell-line-ab264754'>ab264754</a>)

Lane 3:

A549 cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)
  • WB

Supplier Data

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)

All lanes:

Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (ab172974) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Predicted band size: 63 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-DNA Polymerase lambda antibody [EPR7519(2)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7519(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB236985

Anti-DNA Polymerase lambda antibody

Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase lambda antibody (AB236985)

  • 0
  • 0

보완 제품

Primary Antibodies

AB31777

Anti-DNA polymerase alpha/POLA antibody

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

  • 4
  • 3
Primary Antibodies

AB86076

Anti-DNA Polymerase Kappa/POLK antibody

Western blot - Anti-DNA Polymerase Kappa/POLK antibody (AB86076)

  • 0
  • 0
Primary Antibodies

AB157465

Anti-DNA polymerase mu antibody [EPR10470(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-DNA polymerase mu antibody [EPR10470(B)] (AB157465)

  • 0
  • 0
Primary Antibodies

AB264109

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - BSA and Azide free

Recombinant

Flow Cytometry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - BSA and Azide free (AB264109)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DNA polymerase lambda also known as pol λ or DNA pol λ is a DNA polymerase protein involved in DNA repair and replication processes. It has a molecular weight of approximately 65 kDa. DNA polymerase lambda is expressed in various tissues but shows a high concentration in testis and thymus indicating its role in germ cells and immune response. The enzyme exhibits both DNA polymerase and terminal transferase activities facilitating DNA synthesis across gaps and lesions.
Biological function summary

DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.

Pathways

DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.

DNA polymerase lambda is linked to conditions such as cancer and immunodeficiencies where its dysfunction can lead to genomic instability. The loss or aberrant function of DNA pol λ may result in ineffective DNA repair contributing to cancer development due to accumulated DNA damage. It can also interact with tumor suppressor p53 highlighting its involvement in pathways that prevent oncogenesis. Additionally impairments in its function can contribute to immunodeficiency disorders as proper DNA repair is essential for the development and function of the immune system.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

DNA polymerase that functions in several pathways of DNA repair (PubMed : 11457865, PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed : 11457865, PubMed : 19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed : 10887191, PubMed : 10982892, PubMed : 12809503, PubMed : 14627824, PubMed : 15537631, PubMed : 19806195). Also has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed : 11457865, PubMed : 19806195).
See full target information POLL

대체 명칭 보기

DNA polymerase lambda, Pol Lambda, DNA polymerase beta-2, DNA polymerase kappa, Pol beta2, POLL
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 12:e1005893 PubMed27783675

2016

DNA Polymerase κ Is a Key Cellular Factor for the Formation of Covalently Closed Circular DNA of Hepatitis B Virus.

Applications

Unspecified application

Species

Human

Yonghe Qi,Zhenchao Gao,Guangwei Xu,Bo Peng,Chenxuan Liu,Huan Yan,Qiyan Yao,Guoliang Sun,Yang Liu,Dingbin Tang,Zilin Song,Wenhui He,Yinyan Sun,Ju-Tao Guo,Wenhui Li
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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