Anti-DNAJB6 antibody [EPR17122] - N-terminal

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

Intracellular Flow Cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730)IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

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Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 μg.
Lane 2 : ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995)

Predicted band size: 36 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

All lanes:

Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Rat heart lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

RAW264.7 cell lysate at 10 µg

Lane 6:

PC-12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

• WB

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Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

All lanes:

Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (AB198995)

All lanes:

Western blot - Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false