Anti-DNAJC9 antibody [EPR9857] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DNAJC9 antibody [EPR9857] - BSA and Azide free (AB248963)

This data was developed using ab150394, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling DNAJC9 with ab150394 at 1/100 dilution.

• WB

Unknown

Western blot - Anti-DNAJC9 antibody [EPR9857] - BSA and Azide free (AB248963)

This data was developed using ab150394, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DNAJC9 antibody [EPR9857] (<a href='/ko/products/primary-antibodies/dnajc9-antibody-epr9857-ab150394'>ab150394</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 30 kDa

false

• WB

Lab

Western blot - Anti-DNAJC9 antibody [EPR9857] - BSA and Azide free (AB248963)

False colour image of Western blot : Anti-DNAJC9 antibody [EPR9857] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab150394 was shown to bind specifically to DNAJC9. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in DNAJC9 CRISPR-Cas9 edited cell line ab266364 (CRISPR-Cas9 edited cell lysate ab257926). The band observed in the CRISPR-Cas9 edited lysate lane below 35 kDa is likely to represent a truncated form of DNAJC9. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DNAJC9 CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-DNAJC9 antibody [EPR9857] (<a href='/ko/products/primary-antibodies/dnajc9-antibody-epr9857-ab150394'>ab150394</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

DNAJC9 CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human DNAJC9 knockout HEK-293T cell line (<a href='/ko/products/cell-lines/human-dnajc9-knockout-hek-293t-cell-line-ab266364'>ab266364</a>)

Lane 3:

PC-3 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 30 kDa

Observed band size: 35 kDa

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