Anti-DNase gamma antibody [EPR30746-557]

• ELISA

Supplier Data

ELISA - Anti-DNase gamma antibody [EPR30746-557] (AB324943)

Indirect ELISA analysis of ab324943 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse DNase gamma, Mouse DNase I.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with Mouse DNase I.

• WB

Supplier Data

Western blot - Anti-DNase gamma antibody [EPR30746-557] (AB324943)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain kidney, heart (PMID : 10807908).

This blot were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the bands higher than 50 kDa and lower than 20 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DNase gamma antibody [EPR30746-557] (ab324943) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Rat thymus tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-DNase gamma antibody [EPR30746-557] (AB324943)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney, heart (PMID : 10807908).

Lanes 6-8 of this blot were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the bands higher than 50 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DNase gamma antibody [EPR30746-557] (ab324943) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse thymus tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Mouse heart tissue lysate at 20 µg

Lane 6:

A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 8:

AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

true

Exposure time: 180s