Anti-DNase I antibody [EPR28421-50]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody [EPR28421-50] (AB321980)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling DNase I with ab321980 at 1/5000 (0.096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in mouse cardiac muscle. The section was incubated with ab321980 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody [EPR28421-50] (AB321980)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling DNase I with ab321980 at 1/5000 (0.096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in mouse lung. The section was incubated with ab321980 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody [EPR28421-50] (AB321980)

Immunohistochemical analysis of paraffin-embedded Mouse parotid gland tissue labeling DNase I with ab321980 at 1/5000 (0.096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse parotid gland. The section was incubated with ab321980 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNase I antibody [EPR28421-50] (AB321980)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling DNase I with ab321980 at 1/5000 (0.096 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse kidney (A) at 1/1000 dilution (0.478 ug/mL) and (B) at 1/5000 dilution (0.096 ug/mL). The section was incubated with ab321980 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

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Western blot - Anti-DNase I antibody [EPR28421-50] (AB321980)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : heart, cerebellum (PMID : 15015938, PMID : 9192086).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 2 : 10 seconds, lanes 1, 3-5 : 180 seconds

All lanes:

Western blot - Anti-DNase I antibody [EPR28421-50] (ab321980) at 1/1000 dilution

Lane 1:

Mouse small intestine tissue lysate at 20 µg

Lane 2:

Mouse parotid gland tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-DNase I antibody [EPR28421-50] (AB321980)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse DNase IL2 and DNase IL3.

All lanes:

Western blot - Anti-DNase I antibody [EPR28421-50] (ab321980) at 1/1000 dilution

Lane 1:

Mouse DNase I recombinant protein at 10 ng

Lane 2:

Mouse DNase IL2 recombinant protein at 10 ng

Lane 3:

Mouse DNase IL3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 180s