AB240182

Anti-DOCK8 antibody [EPR12511] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal DOCK8 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Dedicator of cytokinesis protein 8, DOCK8

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling DOCK8 with purified ab175208 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Intracellular flow cytometric analysis of permeabilized THP1 cells labeling DOCK8 with ab175208 at 1/10 dilution (red) compared to a Rabbit IgG (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Immunofluorescent staining of Raji cells labeling DOCK8 with ab175208 at 1/100 dilution (green). DAPI nuclear staining (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175208).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12511

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

ab240182 is the carrier-free version of ab175208.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.

Biological function summary

DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.

Pathways

Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.

Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).

See full target information DOCK8

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell 187:4193-4212.e24 PubMed38942014

2024

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Scott-Hewitt,Matthew Mahoney,Youtong Huang,Nils Korte,T Yvanka de Soysa,Daniel K Wilton,Emily Knorr,Kevin Mastro,Allison Chang,Allison Zhang,David Melville,Monica Schenone,Christina Hartigan,Beth Stevens

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DOCK8 antibody [EPR12511] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Flow Cytometry (Intracellular) - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] - BSA and Azide free (AB240182)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise