AB183486

Anti-Dopamine Transporter (phospho T53) antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal Dopamine Transporter phospho T53 antibody. Suitable for WB and reacts with Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc6a3 pT53 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3, Slc6a3

1 이미지

Supplier Data

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (AB183486)

All lanes:

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (ab183486) at 1/1000 dilution

Lane 1:

Rat caudate lysate

Lane 2:

Rat caudate lysate with immunizing phosphopeptide

Predicted band size: 68 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Rat Slc6a3 pT53 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P23977

Specificity

ab183486 is specific for the ~55kDa glycosylated form of the Dopamine Transporter protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the Dopamine Transporter protein. Immunolabeling of the Dopamine Transporter band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab183486 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

보관 버퍼

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mediates sodium- and chloride-dependent transport of dopamine (PubMed : 1502198, PubMed : 1765147, PubMed : 1948034, PubMed : 1948035, PubMed : 8125921). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (PubMed : 8125921). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).

See full target information Slc6a3 pT53

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular psychiatry 29:3607-3622 PubMed38822069

2024

The ion channel TRPA1 is a modulator of the cocaine reward circuit in the nucleus accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jung Kim,Su Jeong Choi,Sa-Ik Hong,Jung-Cheol Park,Youyoung Lee,Shi-Xun Ma,Kwang-Hyun Hur,Young Lee,Kyeong-Man Kim,Hyung Kyu Kim,Hee Young Kim,Seok-Yong Lee,Se-Young Choi,Choon-Gon Jang

CNS neuroscience & therapeutics 29:2608-2620 PubMed37002793

2023

Electroacupuncture alleviated depression-like behaviors in ventromedial prefrontal cortex of chronic unpredictable mild stress-induced rats: Increasing synaptic transmission and phosphorylating dopamine transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Cai,Mei Wu,Zhinan Zhang,Huacong Liu,Shengtao Huang,Jia Song,Siqiang Ren,Yong Huang

International journal of molecular sciences 22: PubMed34502393

2021

Effects of β-Phenylethylamine on Psychomotor, Rewarding, and Reinforcing Behaviors and Affective State: The Role of Dopamine D1 Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

In Soo Ryu,Oc-Hee Kim,Ji Sun Kim,Sumin Sohn,Eun Sang Choe,Ri-Na Lim,Tae Wan Kim,Joung-Wook Seo,Eun Young Jang

British journal of pharmacology 178:3869-3887 PubMed33987827

2021

Methoxphenidine (MXP) induced abnormalities: Addictive and schizophrenia-related behaviours based on an imbalance of neurochemicals in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwang-Hyun Hur,Seong-Eon Kim,Shi-Xun Ma,Bo-Ram Lee,Yong-Hyun Ko,Jee-Yeon Seo,Seon-Kyung Kim,Young-Jung Kim,Su-Jeong Sung,Youyoung Lee,Young Hoon Jung,Yong-Sup Lee,Seok-Yong Lee,Choon-Gon Jang

International journal of molecular sciences 22: PubMed33579026

2021

Prolyl Oligopeptidase Regulates Dopamine Transporter Oligomerization and Phosphorylation in a PKC- and ERK-Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrika H Julku,Maria Jäntti,Reinis Svarcbahs,Timo T Myöhänen

Molecular neurobiology 55:470-482 PubMed27966077

2016

Prolyl Oligopeptidase Regulates Dopamine Transporter Phosphorylation in the Nigrostriatal Pathway of Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrika H Julku,Anne E Panhelainen,Saija E Tiilikainen,Reinis Svarcbahs,Anne E Tammimäki,T Petteri Piepponen,Mari H Savolainen,Timo T Myöhänen

The Journal of biological chemistry 287:29702-12 PubMed22722938

2012

Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux.

Applications

Unspecified application

Species

Unspecified reactive species

James D Foster,Jae-Won Yang,Amy E Moritz,Sathyavathi Challasivakanaka,Margaret A Smith,Marion Holy,Kyle Wilebski,Harald H Sitte,Roxanne A Vaughan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dopamine Transporter (phospho T53) antibody

Western blot - Anti-Dopamine Transporter (phospho T53) antibody (AB183486)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise