AB214179

Anti-DPAGT1/GPT antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal DPAGT1/GPT antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DPAGT1 aa 300-350 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

DPAGT2, DPAGT1, UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, GlcNAc-1-P transferase, N-acetylglucosamine-1-phosphate transferase, G1PT, GPT

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPAGT1/GPT antibody (AB214179)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPAGT1/GPT antibody (AB214179)

Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat brain tissue labeling DPAGT1/GPT with ab214179 at 1/500 dilution, followed by secondary antibody detection and DAB staining.

Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DPAGT1 aa 300-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H3H5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DPAGT1 also known as dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosamine-1-phosphate transferase and sometimes referred to as GPT is an important enzyme in the N-glycosylation pathway. It functions primarily in the endoplasmatic reticulum (ER) of cells. The protein is encoded by the DPAGT1 gene and has a molecular mass typically around 41 kDa. This enzyme is mainly expressed in tissues with high secretory activities such as liver pancreas and placenta where it plays a role in preparing proteins for secretion or membrane localization.

Biological function summary

DPAGT1 initiates the dolichol-linked oligosaccharide synthesis which is critical in protein glycosylation. This protein acts in transferring N-acetylglucosamine (GlcNAc) to dolichol phosphate on the cytoplasmic side of the ER membrane forming GlcNAc-P-P-dolichol an essential precursor for further glycan assembly. DPAGT1 is part of a larger enzyme complex involved in glycoprotein assembly connecting with other enzymes that sequentially build up the oligosaccharide on the dolichol phosphates.

Pathways

Dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosamine-1-phosphate transferase is a catalytic component in the N-glycosylation pathway. This pathway is pivotal for proper folding and function of glycoproteins. N-glycosylation is important for cellular processes like cell-cell recognition and signal transduction. The pathway closely links DPAGT1 with proteins involved in ER-associated degradation (ERAD). DPAGT1 interacts also with downstream glycosyltransferases which further process the glycoproteins for maturation and function.

Dysfunction of DPAGT1 relates to congenital disorders of glycosylation (CDG) particularly CDG type Ij. This disorder arises from improper glycosylation of proteins leading to widespread developmental issues. Dysregulation in DPAGT1 activity can also connect with muscle-eye-brain disease where glycosylation defects affect muscle and ocular tissue development. Disease associations illuminate its interactions with proteins involved in cell differentiation and muscle development pointing to DPAGT1’s broad impact on human health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transferring GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.

See full target information DPAGT1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 15:1500478 PubMed39687617

2024

PCDHGA10 as a potential prognostic biomarker and correlated with immune infiltration in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Zhong,Zhuoqun Yu,Qianqian Wu,Bing Lu,PingPing Sun,Xiaojing Zhang,Lei Yang,Han Wu

PeerJ 12:e16740 PubMed38274331

2024

Regulation of lipid metabolism by APOE4 in intrahepatic cholangiocarcinoma the enhancement of ABCA1 membrane expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiang Qian,Gang Wang,Bin Li,Haoyuan Su,Lei Qin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-DPAGT1/GPT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPAGT1/GPT antibody (AB214179)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise