Anti-Dppa2 antibody [EPR29187-126]

• WB

Supplier Data

Western blot - Anti-Dppa2 antibody [EPR29187-126] (AB324686)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : THP-1, jurkat

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

Lane 5 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-4 : 70 seconds, lane5 : 180 seconds

All lanes:

Western blot - Anti-Dppa2 antibody [EPR29187-126] (ab324686) at 1/1000 dilution

Lane 1:

SW-13 (human adrenal gland cortex epithelial cell) fresh whole cell lysate at 80 µg

Lane 2:

HuTu 80 (human Small intestine Duodenum epithelial) fresh whole cell lysate at 80 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) fresh whole cell lysate at 80 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) fresh whole cell lysate at 80 µg

Lane 5:

NCCIT (human pluripotent embryonic carcinoma epithelial cell) fresh whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Dppa2 antibody [EPR29187-126] (AB324686)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dppa2 antibody [EPR29187-126] (ab324686) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Dppa2 antibody [EPR29187-126] (AB324686)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dppa2 antibody [EPR29187-126] (ab324686) at 1/1000 dilution

Lane 1:

SW-13 (human adrenal gland cortex epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Lane 2:

SW-13 transfected with siRNA specifically targeting DPPA2 whole cell lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Dppa2 antibody [EPR29187-126] (AB324686)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human DPPA4.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Dppa2 antibody [EPR29187-126] (ab324686) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human DPPA2 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human DPPA4 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,100 kDa,36 kDa

false

Exposure time: 3s