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AB237056

Anti-DPY19L3 antibody

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Rabbit Polyclonal DPY19L3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DPY19L3 aa 550-700.

대체 명칭 보기

Protein C-mannosyl-transferase DPY19L3, Dpy-19-like protein 3, Protein dpy-19 homolog 3, DPY19L3

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-DPY19L3 antibody (AB237056)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DPY19L3 antibody (AB237056)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for DPY19L3 using ab237056 at a dilution of 1/200 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DPY19L3 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q6ZPD9

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보완 제품

Primary Antibodies

AB88563

Anti-Synapsin III antibody

Western blot - Anti-Synapsin III antibody (AB88563)

  • 0
  • 0
Primary Antibodies

AB126352

Anti-DPY30 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPY30 antibody (AB126352)

  • 4
  • 1
Primary Antibodies

AB201693

Anti-PIGK antibody [EPR17843]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PIGK antibody [EPR17843] (AB201693)

  • 0
  • 0
Primary Antibodies

AB199178

Anti-Connexin 30.3 / GJB4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 30.3 / GJB4 antibody (AB199178)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DPY19L3 also known as dpy-19-like C-mannosyltransferase 3 is a member of the DPY19 family of proteins. It is expressed in various tissues with significant presence in the testis brain and heart. This protein has a mass of about 75 kDa. DPY19L3 functions as a C-mannosyltransferase an enzyme responsible for transferring mannose to specific tryptophan residues on proteins. Its activity is critical for proper protein glycosylation which affects how proteins fold and maintain stability.
Biological function summary

The function of DPY19L3 plays a vital role in protein modification and localization in cells. It is part of the glycosylation process and interacts with other C-mannosyltransferases to facilitate this process. Glycosylation is essential for protein sorting and function impacting signaling pathways and cellular responses. DPY19L3 often collaborates with DPY19L2 and DPY19L4 which assist in similar enzymatic processes ensuring proteins reach their destinations within the cell.

Pathways

DPY19L3 is integral to N-glycosylation and O-glycosylation pathways. These pathways are essential for producing glycoproteins that have various cellular functions from signaling to immune responses. DPY19L3 interacts with proteins such as mannose transferases and other glycosylation enzymes like MGAT1. Such interactions highlight DPY19L3's importance in generating properly folded and functional proteins including those in immune cell signaling pathways and cell adhesion processes.

DPY19L3 has been associated with fertility issues and certain types of cancer. Alterations in its enzymatic activity can lead to improper protein glycosylation affecting sperm function and contributing to male infertility. Additionally changes in glycosylation patterns due to DPY19L3 mutation may affect cancer cell proliferation and metastasis. This protein is also connected with dysregulated pathways involving proteins like DPY19L2 suggesting potential overlap in disease mechanisms and outcomes between these family members.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins. The reaction occurs on the luminal side of the endoplasmic reticulum and involves the transfer of a mannose unit from a dolichylphosphate mannose (Dol-P-Man) donor to an acceptor protein containing a WxxW or WxxC consensus sequence (PubMed : 26764097, PubMed : 29405629). C-mannosylates RSPO1, a Wnt signaling regulator, preferentially at the first Trp residue in the sequence WxxW (PubMed : 26764097, PubMed : 29405629). C-mannosylates the netrin receptor UNC5A, preferentially at the third tryptophan of WxxWxxWxxC sequence (By similarity).. Isoform 2. Has no C-mannosyltransferase activity.
See full target information DPY19L3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

EXCLI journal 21:1111-1129 PubMed36381643

2022

Expression of connexins and pannexins in diseased human liver.

Applications

Unspecified application

Species

Unspecified reactive species

Kaat Leroy,Vânia Vilas-Boas,Eva Gijbels,Bart Vanderborght,Lindsey Devisscher,Bruno Cogliati,Bert Van Den Bossche,Isabelle Colle,Mathieu Vinken
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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