AB61770

Anti-DREAM antibody

Name: Anti-DREAM antibody
Brand: Abcam Limited
SKU: AB61770
Rating: 4 (1 reviews)

(1 리뷰)

(2 제품이 사용된 논문 )

Rabbit Polyclonal DREAM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KCNIP3.

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (AB61770)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (AB61770)

ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DREAM antibody (AB61770)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DREAM antibody (AB61770)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left : untreated sample. Right : sample treated with immunising peptide.

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Western blot - Anti-DREAM antibody (AB61770)

All lanes:

Western blot - Anti-DREAM antibody (ab61770) at 1/500 dilution

Lane 1:

extracts from A431 cells with no peptide

Lane 2:

extracts from A431 cells with peptide

Predicted band size: 29 kDa

Observed band size: 29 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KCNIP3. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2W7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DREAM also called Downstream Regulatory Element Antagonist Modulator is a calcium-binding protein with a molecular mass of about 29 kDa. It is expressed in various tissues but shows higher levels in the brain and heart. DREAM belongs to the neuronal calcium sensory (NCS) family and acts as a transcriptional repressor that binds to the downstream regulatory element (DRE) sites on DNA. Through its calcium-binding ability DREAM modulates gene expression by changing its conformation depending on intracellular calcium levels.

Biological function summary

Calcium-binding proteins influence multiple cellular processes and DREAM participates in calcium signaling pathways important for neuronal function and gene transcription regulation. As part of the complex interactions within the cell DREAM modulates the expression of target genes by interacting with DRE sequences. Its regulatory role extends to processes including synaptic plasticity neuronal excitability and long-term depression. DREAM's function as a modulator of transcription links it to a broader regulatory network within cells.

Pathways

DREAM engages in calcium signaling pathways and is significant in the transcriptional regulation of genes involved in neuronal processes. DREAM interacts with CREB (cAMP response element-binding protein) and influences downstream gene expression in response to calcium fluctuations impacting the CREB signaling pathway. Additionally DREAM participates in pathways connected to the regulation of pain and inflammatory responses linking to proteins such as NF-kB which plays a role in immune response regulation.

Aberrations in DREAM function associate with neurodegenerative diseases and chronic pain conditions. Its regulatory role in neuronal gene expression connects DREAM to disorders like Alzheimer's and neuropathic pain. In these contexts altered DREAM activity can impact the expression of NF-kB and CREB target genes which are critical in mediating inflammatory responses and neuronal survival. Understanding DREAM's interactions and regulation could therefore provide insights into potential therapeutic targets for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation.

See full target information KCNIP3

대체 명칭 보기

CSEN, DREAM, KCHIP3, KCNIP3, Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, KChIP3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cardiovascular medicine 9:884031 PubMed35711359

2022

Mechanical Strain Induces Transcriptomic Reprogramming of Saphenous Vein Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Davide Maselli,Gloria Garoffolo,Giada Andrea Cassanmagnago,Rosa Vono,Matthijs S Ruiter,Anita C Thomas,Paolo Madeddu,Maurizio Pesce,Gaia Spinetti

Life sciences 227:166-174 PubMed31026452

2019

Vasopressin regulates hypothalamic GnRH synthesis: Histomorphological evidence in hypothalamus and biological effects in GT1-7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zhu,Xiaozhen Zhao,Feng Huang,Feng Wang,Wei Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DREAM antibody

Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (AB61770)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DREAM antibody (AB61770)

Western blot - Anti-DREAM antibody (AB61770)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise