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AB70124

Anti-DUSP22 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal DUSP22 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DUSP22.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-DUSP22 antibody (AB70124)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DUSP22 antibody (AB70124)

ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-DUSP22 antibody (AB70124)
  • WB

Unknown

Western blot - Anti-DUSP22 antibody (AB70124)

All lanes:

Western blot - Anti-DUSP22 antibody (ab70124) at 1/500 dilution

Lane 1:

Jurkat cell extracts at 30 µg

Lane 2:

RAW264.7 cell extracts at 30 µg

Lane 3:

RAW264.7 cell extracts at 30 µg with immunising peptide

Predicted band size: 21 kDa

Observed band size: 20 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human DUSP22. The exact immunogen used to generate this antibody is proprietary information.

Q9NRW4

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대체품

Primary Antibodies

AB150565

Anti-DUSP22 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP22 antibody (AB150565)

  • 0
  • 0

보완 제품

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB129002

Anti-Vinculin antibody [EPR8185] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - Loading Control (AB129002)

  • 5
  • 21
Primary Antibodies

AB203912

Anti-MTCO1 antibody [EPR19628]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)

  • 0
  • 0
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab70124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues (PubMed : 24714587, PubMed : 38225265). Activates the JNK signaling pathway (PubMed : 11717427). Inhibits T-cell receptor signaling and T-cell mediated immune responses, acting, at least in part, by inducing degradation of E3 ubiquitin ligase UBR2 (PubMed : 24714587, PubMed : 38225265). Dephosphorylates and thereby induces 'Lys-48'-linked ubiquitination of UBR2, leading to proteasomal degradation of UBR2 (PubMed : 38225265). Dephosphorylates and thereby inactivates tyrosine kinase LCK (PubMed : 24714587). Inhibits UBR2-mediated 'Lys-63'-linked ubiquitination of LCK (PubMed : 38225265). May play a role in B-cell receptor (BCR) signaling and B-cell function (By similarity).
See full target information DUSP22

대체 명칭 보기

JSP1, LMWDSP2, MKPX, DUSP22, Dual specificity protein phosphatase 22, JNK pathway associated phosphatase, JNK-stimulatory phosphatase-1, Low molecular weight dual specificity phosphatase 2, Mitogen-activated protein kinase phosphatase x, JKAP, JSP-1, LMW-DSP2, MAP kinase phosphatase x, MKP-x
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 24:744 PubMed36561966

2022

Dual specificity phosphatase 22 suppresses mesangial cell hyperproliferation, fibrosis, inflammation and the MAPK signaling pathway in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Na Ren,Shanshan Shi,Na Zhao,Lingyan Zhang

Nature communications 13:5945 PubMed36209205

2022

Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxu Ge,Jun Tan,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Yan Sun,Jing Luo,Chufeng Zhang,Liancai Zhu,Bochu Wang,Minxuan Xu

Theranostics 9:3501-3514 PubMed31281493

2019

Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuanghui Feng,Yuda Wei,Yongxian Zhang,Yan Qiu,Xiaojian Liu,Li Su,Ningning Liang,Huiyong Yin,Qiurong Ding
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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