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AB117515

DyLight® 650 Anti-HA tag antibody [16B12]

4

(1 리뷰)

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(7 제품이 사용된 논문 )

Anti-HA tag antibody [16B12] conjugated to DyLight® 650 - suitable for flow cytometry, ICC/IF samples. Clone [16B12] is a mouse monoclonal antibody cited in 76 publications.

대체 명칭 보기

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

1 이미지
Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)
  • Flow Cyt

AbReview47985****

Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)

ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

Gating Strategy : Gated on FSC-SSC to exclude debris/dead cells.

Grey - unstained cells.

Red - Wild type 293T, stained.

Blue - 293T expressing HA-tagged protein, stained.

This image is courtesy of an anonymous Abreview

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Alkaline Phosphatase

    Alkaline Phosphatase Anti-HA tag antibody [16B12]

  • 518 DyLight® 488

    DyLight® 488 Anti-HA tag antibody [16B12]

  • 576 DyLight® 550

    DyLight® 550 Anti-HA tag antibody [16B12]

  • 615 Europium

    Europium Anti-HA tag antibody [16B12]

  • 578 PE

    PE Anti-HA tag antibody [16B12]

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

16B12

Isotype

IgG1

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Carrier free

No

Applications

ICC/IF, Flow Cyt

applications

Specificity

<p>ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p></p>" } } }

제품 세부 정보

Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 95% Sodium phosphate, 0.58% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
The product can be stored for up to 12 months

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules 13: PubMed37189383

2023

Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique Heymann

Cell reports 33:108249 PubMed33053346

2020

Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ildar Gabaev,James C Williamson,Thomas W M Crozier,Thomas F Schulz,Paul J Lehner

Cell reports 31:107691 PubMed32460028

2020

Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Nafria,Peter Keane,Elizabeth S Ng,Edouard G Stanley,Andrew G Elefanty,Constanze Bonifer

The Journal of cell biology 219: PubMed32344433

2020

GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508.

Applications

Unspecified application

Species

Unspecified reactive species

Safa Lucken-Ardjomande Häsler,Yvonne Vallis,Mathias Pasche,Harvey T McMahon

eLife 9: PubMed32292164

2020

Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Marelli,James C Williamson,Anna V Protasio,Adi Naamati,Edward Jd Greenwood,Janet E Deane,Paul J Lehner,Nicholas J Matheson

Scientific reports 7:13448 PubMed29044191

2017

LRRC25 Functions as an Inhibitor of NF-κB Signaling Pathway by Promoting p65/RelA for Autophagic Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Feng,Tianhao Duan,Yang Du,Shouheng Jin,Mingjun Wang,Jun Cui,Rong-Fu Wang

eLife 5: PubMed27690223

2016

Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.

Applications

Unspecified application

Species

Unspecified reactive species

Edward Jd Greenwood,Nicholas J Matheson,Kim Wals,Dick Jh van den Boomen,Robin Antrobus,James C Williamson,Paul J Lehner
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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