AB248734

Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Dynamin 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

KIAA0820, DNM3, Dynamin-3, T-dynamin

1 이미지

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Western blot - Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free (AB248734)

This data was developed using ab134925, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dynamin 3 antibody [EPR9055] (<a href='/ko/products/primary-antibodies/dynamin-3-antibody-epr9055-ab134925'>ab134925</a>) at 1/1000 dilution

All lanes:

Fetal brain lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9055

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab248734 is the carrier-free version of ab134925.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Dynamin 3 also known as DNM3 is a GTPase enzyme involved in the regulation of membrane fission events. It belongs to the dynamin family which also includes dynamin 1 and dynamin 2. Dynamin 3 has a molecular weight of approximately 97 kDa. This protein expresses in neurons where it plays a critical role in synaptic vesicle endocytosis. Additionally its expression can be found in the testes and lungs indicating a role in cellular events specific to these tissues.

Biological function summary

Dynamin 3 functions as part of multi-protein complexes that mediate membrane trafficking. In neurons it partners with other proteins to ensure precise timing of vesicle scission during synaptic transmission. Its ability to hydrolyze GTP provides the force necessary for membrane constriction and this activity is essential for maintaining normal neurotransmitter release. Dynamin 3 interacts with proteins including endophilin and amphiphysin which assist in the binding to membrane lipids ensuring proper recruitment to the sites of vesicle budding.

Pathways

Cellular events involving dynamin 3 are integral to synaptic vesicle recycling and endocytosis. Dynamin 3 functions within the clathrin-mediated endocytosis pathway which ensures the recycling of synaptic vesicles following neurotransmitter release. Within this pathway it collaborates extensively with clathrin and adaptin proteins playing a critical role in the disassembly of the clathrin-coated pits. Dynamin 3's activity is closely coordinated with other members of the dynamin protein family such as dynamin 1 which shares overlapping roles in similar cellular contexts.

Dysregulation of dynamin 3 is associated with certain neurodegenerative disorders such as Charcot-Marie-Tooth disease and epilepsy. Alterations in its function can disrupt normal synaptic vesicle trafficking leading to impaired synaptic transmission contributing to the pathology of these conditions. The interaction of dynamin 3 with proteins such as myelin protein zero (related to Charcot-Marie-Tooth disease) and voltage-gated ion channels (related to epilepsy) highlights its significant role in the nervous system where its proper function is needed for cognitive and motor abilities.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).

See full target information DNM3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free

Western blot - Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free (AB248734)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise