Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971)

This data was developed using ab287970, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) whole cell lysate labeling E Cadherin with ab287970 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab287970 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/500 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150117) secondary antibody at 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971)

This data was developed using ab287970, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

Full-length E-cadherin is ~120 kDa. The other bands are due to proteolytic cleavage in different Cadherin domains (PMID : 11112695; PMID : 11212238).

All lanes:

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (<a href='/ko/products/primary-antibodies/e-cadherin-antibody-36-e-cadherin-ab287970'>ab287970</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 110 kDa,120 kDa

false

Exposure time: 7s

• WB

Supplier Data

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971)

This data was developed using ab287970, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

This antibody has cross-reactivity with P-Cadherin.

All lanes:

Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (<a href='/ko/products/primary-antibodies/e-cadherin-antibody-36-e-cadherin-ab287970'>ab287970</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with P-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with E-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 120 kDa

false

Exposure time: 3s