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AB242673

Anti-E Cadherin antibody [EPR16845-141] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat, Human samples.
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Sandwich ELISA - Anti-E Cadherin antibody [EPR16845-141] - BSA and Azide free (Capture) (AB242673)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-E Cadherin antibody [EPR16845-141] - BSA and Azide free (Capture) (AB242673)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233611).

Sandwich ELISA - Anti-E Cadherin antibody [EPR16845-141] - BSA and Azide free (Capture) (AB242673)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-E Cadherin antibody [EPR16845-141] - BSA and Azide free (Capture) (AB242673)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab197751).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16845-141

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB233611

Human E-Cadherin ELISA Kit

Recombinant|SimpleStep

ELISA - Human E-Cadherin ELISA Kit (AB233611)

  • 5
  • 1
Sample Prep & Detection Kits

AB195225

Texas Red® Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Texas Red® Conjugation Kit (Fast) - Lightning-Link® (AB195225)

  • 0
  • 0
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Primary Antibodies

AB267436

Anti-LIX antibody [EPR22392-51] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-LIX antibody [EPR22392-51] - BSA and Azide free (Detector) (AB267436)

  • 0
  • 0
Primary Antibodies

AB242709

Anti-CXCL2 antibody [EPR17014-241] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL2 antibody [EPR17014-241] - BSA and Azide free (Detector) (AB242709)

  • 0
  • 0
Primary Antibodies

AB259472

Anti-MIP-3 beta/CCL19 antibody [EPR21931-34] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-MIP-3 beta/CCL19 antibody [EPR21931-34] - BSA and Azide free (Detector) (AB259472)

  • 0
  • 0
Primary Antibodies

AB259490

Anti-EpCAM antibody [EPR20533-1] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-EpCAM antibody [EPR20533-1] - BSA and Azide free (Detector) (AB259490)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242673 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse/Rat E-Cadherin. The recommended pair for sandwich ELISA is:
Capture: ab242673, Mouse/Rat E-Cadherin Capture Antibody (unconjugated)
Detector: ab242794, Mouse/Rat E-Cadherin Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml. for mouse and 62.5 - 4000 pg/ml for rat.

Or alternatively, for Human, the recommended pair for sandwich ELISA is:
Capture: ab242673, Human E-Cadherin Capture Antibody (unconjugated)
Detector: ab244997, Human E-Cadherin Detector Antibody (unconjugated)
The reference range value is 156 - 10000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
See full target information CDH1

대체 명칭 보기

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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