AB238099

Anti-E Cadherin antibody [rCDH1/1525]

Recombinant
Lab Essentials
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(22 제품이 사용된 논문 )

Mouse Recombinant Monoclonal E Cadherin antibody. Suitable for IHC-P, Protein Array, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH1.

대체 명칭 보기

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for E Cadherin with ab238099 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

Protein Array

Unknown

Protein Array - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

ab238099 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supplier Data

Western blot - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

All lanes:

Western blot - Anti-E Cadherin antibody [rCDH1/1525] (ab238099) at 1 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 97 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

rCDH1/1525

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, Protein Array, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDH1.

P12830

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.", "ProteinArray-species-checked": "notRecommended", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/µL", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from bioreactor concentrate.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1

style

left-column

제품이 사용된 논문 (22)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Open life sciences 19:20220990 PubMed39759103

2025

MFAP5 inhibits the malignant progression of endometrial cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Liang,Zijuan Qi,Chun Du

Journal of biochemical and molecular toxicology 38:e23769 PubMed39152098

2024

Cancer-associated fibroblasts derived exosomal LINC01833 promotes the occurrence of non-small cell lung cancer through miR-335-5p -VAPA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jian-Jun Sun,Ya-Wen Ma,Meng-Qin Zhu,Jing Hu,Qi-Jue Lu,Zhi-Gang Cai

Cell adhesion & migration 18:1-12 PubMed38831518

2024

Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Yang,Xiaoni Zhong,Xiaoling Gao,Wenyi Xie,Yaokai Chen,Yuanjiang Liao,Peilin Zhang

Heliyon 10:e31520 PubMed38828336

2024

ANGPTL3 diminishes the resistance of ovarian cancer to paclitaxel by blocking the PI3K-AKT-mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wu,Jia Liu,Xin Yang,Zhifen Wu,Tingzhao Wang,Meiqin Xiao

Journal of biochemical and molecular toxicology 38:e23705 PubMed38602237

2024

CircNRD1 elevates THAP domain containing 11 through sequestering microRNA-421 to inhibit gastric cancer growth and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anwen Liu,Jingcong Liang,Jianfeng Wen

International journal of biological sciences 20:818-830 PubMed38250159

2024

IGF2BP2 promotes cell invasion and epithelial-mesenchymal transition through Src-mediated upregulation of EREG in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chiao-Wen Lin,Wei-En Yang,Chun-Wen Su,Hsueh-Ju Lu,Shih-Chi Su,Shun-Fa Yang

The Prostate 84:317-328 PubMed38145367

2023

PrLZ regulates EMT and invasion in prostate cancer via the TGF-β1/p-smad2/miR-200 family/ZEB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Xie,Jiaqi Chen,Zhenkun Ma,Yang Gao,Jin Zeng,Yule Chen,Zhao Yang,Shan Xu

Histology and histopathology 39:497-509 PubMed37702425

2023

miR-638 suppresses cervical cancer progression by inhibiting under the treatment of Tetrandrine.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Kai Liu,Zhongming Zhou,Huizhuan Geng

Journal of viral hepatitis 30:819-829 PubMed37421213

2023

Depletion of circ_0088046 suppressed cell growth and motility of hepatocellular carcinoma via circ_0088046-miR-1299-RTKN2 ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Sai He,Hao Guan,Yao Zhao,Di Zhang

Journal of microbiology and biotechnology 33:398-409 PubMed36655275

2023

LncRNA LINC01232 Enhances Proliferation, Angiogenesis, Migration and Invasion of Colon Adenocarcinoma Cells by Downregulating miR-181a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yuan,Zhou Long

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin antibody [rCDH1/1525]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

Protein Array - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

Western blot - Anti-E Cadherin antibody [rCDH1/1525] (AB238099)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (22)

Product promise