AB76319

Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]

Name: Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]
Brand: Abcam Limited
SKU: AB76319
Rating: 5 (2 reviews)

BOND RX™ Validated
RabMAb
Recombinant
Lab Essentials
20ul selling size
각 태그의 뜻

(2 리뷰들)

(83 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal E Cadherin phospho S838 + S840 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 83 publications.

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling E Cadherin with Purified ab76319 at 1 : 80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling E Cadherin with Purified ab76319 at 1 : 80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

AbReview44796****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Unpurified ab76319 staining E Cadherin in mouse skin (pilosebaceous untis) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 10% normal donkey serum + 1% serum for 40 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. An Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling E Cadherin with Purified ab76319 at 1 : 80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution

Lane 1:

Human brain lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 2:

Human brain lysate at 15 µg

Lane 3:

Rat brain lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 4:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

Observed band size: 145 kDa

false

Unknown

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/10000 dilution

All lanes:

Mouse brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 97 kDa

Observed band size: 145 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP913(2)Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified use at 1/500000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/80000", "IHCP-species-notes": "For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified use at 1/500000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/80000", "IHCP-species-notes": "For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified use at 1/500000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/80000", "IHCP-species-notes": "For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1 pS838 + S840

대체 명칭 보기

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

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제품이 사용된 논문 (83)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Research (Washington, D.C.) 8:0864 PubMed40904624

2025

Ligustilide Suppresses Macrophage-Mediated Intestinal Inflammation and Restores Gut Barrier via EGR1-ADAM17-TNF-α Pathway in Colitis Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyang Li,Yequn Wu,Jing Liang,Peiqi Chen,Shihua Xu,Yumei Wang,Zhi Jiang,Xudong Zhu,Chaozhan Lin,Yang Yu,Hailin Tang

Histology and histopathology :18974 PubMed40772306

2025

Suppressing SMURF1 to preserve GSTM2: An approach to reducing gastric cancer aggressiveness and .

Applications

Unspecified application

Species

Unspecified reactive species

Guoming Ma,Jingwu Li,Yuanting Lu,Weiwei Zuo

Communications biology 8:975 PubMed40595410

2025

circGIGYF1 inhibits stemness and metastasis in colorectal cancer by promoting WWP2-HOXD13 interaction to regulate β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhao,Jiacheng Li,Zunxian Wang,Hairui Zhou,Zhuoxin Cheng,Shuxia Ma

Scientific reports 15:5703 PubMed39962178

2025

Knockdown of CCNB2 inhibits the tumorigenesis of gastric cancer by regulation of the PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Hu,Pengxian Tao,Yongfeng Wang,Chengzhang Zhu,Yuntao Ma,Xiaolong Liu,Hui Cai

Journal of biochemical and molecular toxicology 38:e70080 PubMed39635830

2024

Exosomal miR-20b-5p Induces EMT and Enhances Progression in Non-Small Cell Lung Cancer Via TGFBR2 Downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ma,Bo Jiang,Qiu Ren,Yajiao Sun,Mengyao Wang,Siyu Xia,Dong Wang,Wei Zhang

Cell reports 43:114837 PubMed39368089

2024

Volumetric analysis of the terminal ductal lobular unit architecture and cell phenotypes in the human breast.

Applications

Unspecified application

Species

Unspecified reactive species

Oona Paavolainen,Markus Peurla,Leena M Koskinen,Jonna Pohjankukka,Kamyab Saberi,Ella Tammelin,Suvi-Riitta Sulander,Masi Valkonen,Larissa Mourao,Pia Boström,Nina Brück,Pekka Ruusuvuori,Colinda L G J Scheele,Pauliina Hartiala,Emilia Peuhu

Heliyon 10:e36340 PubMed39253270

2024

M1 macrophages as promising agents for cell therapy of endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Artemova,Polina Vishnyakova,Andrey Elchaninov,Elena Gantsova,Gennady Sukhikh,Timur Fatkhudinov

Discover oncology 15:398 PubMed39222250

2024

Hsa_circ_ROBO1 competes with miR-324-3p and upregulates NME1 to promote tumor metastasis in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

FenFeng Liang,Hai Zhao,GengChun Liu,MeiLing Huang,ZhengJia Peng

BMC oral health 24:518 PubMed38698370

2024

F. Nucleatum enhances oral squamous cell carcinoma proliferation via E-cadherin/β-Catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrui Li,Yuan Liu,Xufeng Huang,Qi Wang,Rao Fu,Xutao Wen,Ji'an Liu,Ling Zhang

Oncology reports 51: PubMed38391012

2024

Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Junyi Wu,Huiqin Zhu,Xiaoqin Shi,Jun Liu,Yang Li,Min Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (83)

Product promise