Anti-E2F2 antibody [EPR27391-170]

• IP

Supplier Data

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (AB318956)

E2F2 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Ramos whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (ab318956) at 1/30 dilution

All lanes:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (AB318956)

E2F2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Raji whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (ab318956) at 1/30 dilution

All lanes:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] (AB318956)

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] (AB318956)

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-E2F2 antibody [EPR27391-170] (AB318956)

Chromatin was prepared from Raji cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab318956 [EPR27391-170]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 60 million reads. The Input control is also shown.

• WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] (AB318956)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (ab318956) at 1/1000 dilution

Lane 1:

Human lung cancer tissue lysate at 50 µg

Lane 2:

Human liver cancer tissue lysate at 50 µg

Lane 3:

Human placenta tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] (AB318956)

Low expression : K-562, 293T.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (ab318956) at 1/1000 dilution

Lane 1:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 50 µg

Lane 3:

Ramos (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-E2F2 antibody [EPR27391-170] (AB318956)

This antibody does not cross-react with human E2F3.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-E2F2 antibody [EPR27391-170] (ab318956) at 1/1000 dilution

Lane 1:

His-tagged human E2F2 recombinant protein at 10 ng

Lane 2:

His-tagged human E2F3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa

false

Exposure time: 180s