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AB314180

Anti-E2F4 antibody [24E12]

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Rabbit Recombinant Monoclonal E2F4 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human E2F4.

대체 명칭 보기

Transcription factor E2F4, E2F-4, E2F4

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [24E12] (AB314180)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [24E12] (AB314180)

Immunohistochemical analysis of ab314180 diluted at 1 : 100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Flow Cytometry (Intracellular) - Anti-E2F4 antibody [24E12] (AB314180)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-E2F4 antibody [24E12] (AB314180)

Overlay Peak curve showing MCF7 (Human breast adenocarcinoma cell line) cells stained with ab314180 (pink line) at 1 : 100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1x10E6cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1 : 200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1x10E6cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-E2F4 antibody [24E12] (AB314180)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E2F4 antibody [24E12] (AB314180)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314180 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor® 513-conjugated Goat Anti-Rabbit IgG (H+L).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

24E12

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human E2F4.

Q16254

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보완 제품

Primary Antibodies

AB76255

Anti-p130 (phospho S672) antibody [EP2126Y]

RabMAb

Immunoprecipitation - Anti-p130 (phospho S672) antibody [EP2126Y] (AB76255)

  • 0
  • 0
Primary Antibodies

AB109596

Anti-E2F8 antibody

Immunoprecipitation - Anti-E2F8 antibody (AB109596)

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Primary Antibodies

AB262931

Anti-LIN52 antibody

Immunocytochemistry/ Immunofluorescence - Anti-LIN52 antibody (AB262931)

  • 0
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Primary Antibodies

AB76234

Anti-p130 antibody [EP2141Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] (AB76234)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/200", "FlowCytIntra-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

E2F4 sometimes known as E2F-4 is part of the E2F family of transcription factors. It plays a critical role in controlling the cell cycle by regulating the expression of genes necessary for DNA synthesis. E2F4 has a molecular weight of around 45 kDa. These transcription factors are widely expressed in a variety of tissues reflecting their importance in cell biology. E2F4 typically functions by forming complexes with dimerization partner (DP) proteins mainly in the nucleus where it binds to DNA and influences transcription.
Biological function summary

E2F4 functions as a regulator of cell cycle progression. It forms complexes localizing mostly in the nucleus controlling the transition from G1 to S phase by regulating genes associated with cell cycle arrest and DNA replication. Partnering with members of the DP family enhances its DNA binding capacity influencing genes involved in cell cycle arrest and DNA repair processes. E2F4 predominantly acts as a transcriptional repressor in quiescent and differentiating cells maintaining the cell in a non-proliferative state.

Pathways

E2F4 is significant in the regulation of the cell cycle and DNA damage response pathways. It engages with the retinoblastoma protein family (pRB p107 p130) to exert repressive effects on E2F-target genes ensuring proper cell cycle exit and maintaining the G0 phase. The interaction with p130 is instrumental during the G0 state preventing inappropriate cell proliferation and contributing to the maintenance of cellular quiescence.

E2F4's role is noteworthy in cancer and retinoblastoma. Alterations in its regulation or activity can lead to unchecked cell proliferation a hallmark of cancerous growths. The malfunction or suppression of its activity can disrupt cell cycle control contributing to oncogenesis. In instances of retinoblastoma improper interaction between E2F4 and the retinoblastoma protein family can result in disrupted cell cycle checkpoints highlighting its importance in maintaining cellular homeostasis and preventing tumorigenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation : together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
See full target information E2F4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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