Anti-EAAT1 antibody

• WB

Unknown

Western blot - Anti-EAAT1 antibody (AB416)

All lanes:

Western blot - Anti-EAAT1 antibody (ab416)

All lanes:

Rat brain cortex

Predicted band size: 59 kDa

Observed band size: 150 kDa,60 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (AB416)

ab416 (1 : 500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• WB

CiteAb

Western blot - Anti-EAAT1 antibody (AB416)

EAAT1 western blotting using Anti-EAAT1 antibody ab416. Publication image and figure legend from Sidoryk-Wegrzynowicz, M., Gerber, Y. N., et al., 2017, Acta Neuropathol Commun, Pubmed 29187256.

Astrocytes from P301S mice express more GFAP and S100β and less GS, GLT-1 and GLAST than astrocytes from control mice. a, d Representative blots of astrocyte-specific protein markers related to gliosis/proliferation (GFAP, S100β) and function (glutamine synthetase (GS) and the glutamate transporters (GLT-1 and GLAST)) in the superficial motor cortex of 3 month-old and 5 month-old C57 and P301S mice. h Expression of the same markers in primary pure cultured astrocytes from 7 day-old mice after days 8 in vitro (98% culture confluence). Mean ± SEM, *p < < 0.05 vs control; unpaired t test, N = 3 independent experiments (mice : GFAP, S100β (b, c); GLT-1, GLAST, GS (e-g); primary cultures i). Vertical lines in (d) denote point were the picture of the Western blot was assembled from two parts cut from the same blot

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