AB247650

Anti-EBF1 antibody [EPR4183] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal EBF1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

대체 명칭 보기

COE1, EBF, EBF1, Transcription factor COE1, O/E-1, OE-1, Early B-cell factor

2 이미지

Unknown

Western blot - Anti-EBF1 antibody [EPR4183] - BSA and Azide free (AB247650)

We are unsure as to the identity of the extra bands.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108369).

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (<a href='/ko/products/primary-antibodies/ebf1-antibody-epr4183-ab108369'>ab108369</a>) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Mouse spleen lysate at 20 µg

Lane 4:

Mouse lymph node lysate at 20 µg

Lane 5:

Rat spleen lysate at 20 µg

Lane 6:

Rat lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Lab

Western blot - Anti-EBF1 antibody [EPR4183] - BSA and Azide free (AB247650)

This data was developed using ab108369, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.

All lanes:

Western blot - Anti-EBF1 antibody [EPR4183] (<a href='/ko/products/primary-antibodies/ebf1-antibody-epr4183-ab108369'>ab108369</a>) at 1/1000 dilution

Lane 1:

Mouse spleen frozen lysate at 20 µg

Lane 2:

Mouse spleen fresh lysate at 20 µg

Lane 3:

Rat spleen frozen lysate at 20 µg

Lane 4:

Rat spleen fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4183

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody also recognises EBF3 protein.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

ab247650 is the carrier-free version of ab108369.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EBF1 also known as Early B-cell Factor 1 is a transcription factor with a molecular mass of approximately 65 kDa. This protein plays a critical role in the development and differentiation of B-cells. EBF1 is expressed mainly in developing B-cells as well as some neurons indicating its role in the immune and nervous systems. The protein contains a helix-loop-helix motif which helps it bind to DNA and modulate gene expression.

Biological function summary

Early B-cell Factor 1 serves as a major regulator during B-cell development. It contributes to the transcriptional network essential for the maturation of progenitor cells into fully functional B-cells. Although not typically found in a larger complex EBF1 interacts with other transcription factors to ensure proper lineage differentiation and function. Its activity influences the expression of various genes important for immune response.

Pathways

Early B-cell Factor 1 connects deeply with the B-cell receptor signaling pathway which is essential for B-cell survival and proliferation. EBF1 often interacts through this pathway with proteins like PAX5 which also plays an important role in B-cell lineage commitment. Additionally EBF1 can interact with the Notch signaling pathway which affects cellular differentiation processes during development.

Dysregulation of Early B-cell Factor 1 activity often associates with immunological diseases such as acute lymphoblastic leukemia (ALL) and B-cell lymphomas. Aberrant expression levels or mutations in EBF1 can disrupt normal B-cell functioning and lead to such malignancies. EBF1 is linked intricately with proteins like TCF3 where aberrations in both can lead to leukemic conditions emphasizing its role in maintaining cellular signaling balance in immune-related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Key pioneer transcription factor of B-cell specification and commitment (PubMed : 27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).. (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed : 28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed : 26819314).

See full target information EBF1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EBF1 antibody [EPR4183] - BSA and Azide free

Western blot - Anti-EBF1 antibody [EPR4183] - BSA and Azide free (AB247650)

Western blot - Anti-EBF1 antibody [EPR4183] - BSA and Azide free (AB247650)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise