AB137403

Anti-EEA1 antibody - C-terminal

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(4 제품이 사용된 논문 )

Rabbit Polyclonal EEA1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 1300 to C-terminus.

대체 명칭 보기

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells labelling EEA1 with ab137403 at 1/500 (Green). Alpha-tubulin filaments are labeled in Red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling EEA1 protein using ab137403 at a 1/500 dilution (Green). Beta Tubulin 3/ Tuj1 was counterstained in red. DAPI was used for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for EEA1 protein at cytosol on mouse brain with ab137403 at a 1/500 dilution.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

Unknown

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

5% SDS PAGE

All lanes:

Western blot - Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 162 kDa

false

Supplier Data

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 160 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EEA1 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15075

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.

Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

See full target information EEA1

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38612903

2024

Characterization of the First Secreted Sorting Nexin Identified in the Protists.

Applications

Unspecified application

Species

Unspecified reactive species

Olympia Tziouvara,Marina Petsana,Drosos Kourounis,Amalia Papadaki,Efthimia Basdra,Georgia G Braliou,Haralabia Boleti

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 257:83-94 PubMed30367290

2018

Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Heidrun L Deissler,Gerhard K Lang,Gabriele E Lang

Oncotarget 9:6463-6477 PubMed29464085

2018

Intravesicular epidermal growth factor receptor subject to retrograde trafficking drives epidermal growth factor-dependent migration.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Maisel,Derrick Broka,Joyce Schroeder

Experimental eye research 143:49-59 PubMed26481553

2015

Internalization of bevacizumab by retinal endothelial cells and its intracellular fate: Evidence for an involvement of the neonatal Fc receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Heidrun L Deissler,Gerhard K Lang,Gabriele E Lang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEA1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - C-terminal (AB137403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEA1 antibody - C-terminal (AB137403)

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

Western blot - Anti-EEA1 antibody - C-terminal (AB137403)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise