AB206860

Anti-EEA1 antibody - Early Endosome Marker

Name: Anti-EEA1 antibody - Early Endosome Marker
Brand: Abcam Limited
SKU: AB206860
Rating: 5 (1 reviews)

Lab Essentials
각 태그의 뜻

(1 리뷰)

(8 제품이 사용된 논문 )

Goat Polyclonal EEA1 antibody. Early Endosome marker. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EEA1 aa 800-850.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining EEA1. Primary incubation with ab206860 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.

Supplier Data

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

All lanes:

Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab206860) at 0.3 µg/mL

All lanes:

HEK293 lysate (in RIPA buffer) at 35 µg

Predicted band size: 162 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EEA1 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

Q15075

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab206860 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.

Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

See full target information EEA1

대체 명칭 보기

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록and refine your search

Nature nanotechnology 20:683-696 PubMed39984637

2025

Systemic HER3 ligand-mimicking nanobioparticles enter the brain and reduce intracranial tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Alonso-Valenteen,Simoun Mikhael,HongQiang Wang,Jessica Sims,Michael Taguiam,James Teh,Sam Sances,Michelle Wong,Tianxin Miao,Dustin Srinivas,Nelyda Gonzalez-Almeyda,Ryan H Cho,Romny Sanchez,Kimngan Nguyenle,Erik Serrano,Briana Ondatje,Rebecca L Benhaghnazar,Harry B Gray,Zeev Gross,John Yu,Clive N Svendsen,Ravinder Abrol,Lali K Medina-Kauwe

International journal of molecular sciences 25: PubMed39769151

2025

Keratinocyte-Mediated Antigen Presentation in Psoriasis: Preliminary Insights from In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Zima,Dorota Purzycka-Bohdan,Aneta Szczerkowska-Dobosz,Magdalena Gabig-Cimińska

Cell reports 40:111062 PubMed35830807

2022

Inhibition of SIRT2 promotes APP acetylation and ameliorates cognitive impairment in APP/PS1 transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Bai,Na Li,Rong Cheng,Yi Guan,Xiong Zhao,Zhijie Song,Hongde Xu,Fei Yi,Bo Jiang,Xiaoman Li,Xuan Wu,Cui Jiang,Tingting Zhou,Qiqiang Guo,Wendong Guo,Yanling Feng,Zhuo Wang,Mengtao Ma,Yang Yu,Zhanyou Wang,Shengping Zhang,Chuangui Wang,Weidong Zhao,Shihui Liu,Xiaoyu Song,Hua Liu,Liu Cao

JHEP reports : innovation in hepatology 4:100391 PubMed34917912

2021

Immunomodulatory receptor VSIG4 is released during spontaneous bacterial peritonitis and predicts short-term mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Reißing,Philipp Lutz,Mick Frissen,Oluwatomi Ibidapo-Obe,Philipp A Reuken,Theresa H Wirtz,Sven Stengel,Stefanie Quickert,Michael Rooney,Karsten Große,Henning W Zimmermann,Christian Trautwein,Andreas Stallmach,Tony Bruns

Advanced healthcare materials 11:e2102027 PubMed34865307

2021

Harnessing the Natural Healing Power of Colostrum: Bovine Milk-Derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Hyosuk Kim,Da Eun Kim,Geonhee Han,Nu Ri Lim,Eun Hye Kim,Yeongji Jang,Haeun Cho,Hochung Jang,Ki Hun Kim,Sun Hwa Kim,Yoosoo Yang

Frontiers in pharmacology 12:710722 PubMed34603025

2021

Intervention of Gastrodin in Type 2 Diabetes Mellitus and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Bai,Ke Mo,Guirong Wang,Wanling Chen,Wei Zhang,Yibo Guo,Zhirong Sun

mSphere :e0021721 PubMed34133202

2021

Cold Atmospheric Plasma Promotes Killing of Staphylococcus aureus by Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Constance Duchesne,Nadira Frescaline,Océane Blaise,Jean-Jacques Lataillade,Sébastien Banzet,Olivier Dussurget,Antoine Rousseau

Nucleic acids research 47:11020-11043 PubMed31617560

2019

HER3-targeted protein chimera forms endosomolytic capsomeres and self-assembles into stealth nucleocapsids for systemic tumor homing of RNA interference in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Alonso-Valenteen,Sayuri Pacheco,Dustin Srinivas,Altan Rentsendorj,David Chu,Jay Lubow,Jessica Sims,Tianxin Miao,Simoun Mikhael,Jae Youn Hwang,Ravinder Abrol,Lali K Medina Kauwe

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EEA1 antibody - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB206860)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (8)

Product promise