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AB235235

Anti-EEF1A2 antibody

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Goat Polyclonal EEF1A2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

대체 명칭 보기

EEF1AL, STN, EEF1A2, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, Statin-S1, eEF1A-2

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB235235)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB235235)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EEF1A2 with ab235235 at 1/100 dilution (30 minutes at RT), followed by a Donkey anti-Goat (HRP) (4 μl/ml, 20 mins at RT). Counterstained with hematoxylin.

Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

Top panel : Human skeletal muscle H&E.

Bottom left panel : ab235235 at 20X magnification. Bottom right panel : ab235235 at 40X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-EEF1A2 antibody (AB235235)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EEF1A2 antibody (AB235235)

Immunofluorescent analysis of 100% methanol-fixed, 0.3% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EEF1A2 with ab235235 at 15 μg/ml (Overnight at 4°C), followed by anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 μg/ml (1 hour at RT) (green).

Expected localization : nucleoli, cytosol.

Western blot - Anti-EEF1A2 antibody (AB235235)
  • WB

Supplier Data

Western blot - Anti-EEF1A2 antibody (AB235235)

All lanes:

Western blot - Anti-EEF1A2 antibody (ab235235) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 35 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) nuclear extract lysate at 35 µg

Lane 3:

Mouse adult brain lysate at 35 µg

Lane 4:

Mouse liver lysate at 35 µg

Secondary

All lanes:

Goat IgG (H&L) antibody peroxidase conjugate at 1/5000 dilution

Predicted band size: 50 kDa

false

Exposure time: 6s

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB235190

Anti-EEF1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB235190)

  • 0
  • 0
Primary Antibodies

AB212172

Anti-EEF1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB212172)

  • 0
  • 0
Primary Antibodies

AB256523

Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] - BSA and Azide free (AB256523)

  • 0
  • 0
Primary Antibodies

AB227275

Anti-EEF1A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1A2 antibody (AB227275)

  • 0
  • 0
Primary Antibodies

AB227824

Anti-EEF1A2 antibody [EPR22651-46]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-EEF1A2 antibody [EPR22651-46] (AB227824)

  • 0
  • 0

보완 제품

Primary Antibodies

AB93607

Anti-SHANK3 antibody [S69]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [S69] (AB93607)

  • 3
  • 1
Primary Antibodies

AB157455

Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471] (AB157455)

  • 4
  • 1
Primary Antibodies

AB307728

Anti-eIF4A2 antibody [EPR27347-74]

BOND RX™ Validated|20ul selling size|KO Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A2 antibody [EPR27347-74] (AB307728)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "100 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EF1A2 also known as Eukaryotic translation elongation factor 1 alpha 2 is a protein that plays a role in the elongation phase of protein synthesis. It is a GTP-binding protein with a mass of about 50 kDa. This protein facilitates the binding of aminoacyl-tRNA to the acceptor site of the ribosome during protein translation. EF1A2 is expressed mainly in heart brain and skeletal muscle tissues indicating its importance in tissues with high energy demands.
Biological function summary

EF1A2 supports cellular proliferation and growth by ensuring the continuous efficiency of protein synthesis. It participates in several protein complexes that are involved in translational control. By regulating the actin cytoskeleton EF1A2 additionally influences cell shape and motility. This multifunctionality indicates EF1A2's relevance in various cellular activities beyond its primary role in translation elongation.

Pathways

EF1A2 integrates into the protein synthesis pathway and also plays a role in the mTOR signaling pathway which regulates cell growth and metabolism. In the mTOR pathway EF1A2 works alongside other elongation factors like EF2 and components of the translation machinery to coordinate protein synthesis. It interacts with proteins involved in nutrient signaling and energy status showing its role in integrating metabolic signals with translational processes.

EF1A2 shows a connection with cancer and neurodegenerative diseases. Overexpression of EF1A2 has been linked to several types of cancer as its role in facilitating protein synthesis can contribute to uncontrolled cellular proliferation. In neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) altered expression of EF1A2 suggests a disruption in protein homeostasis. The dysregulation of EF1A2 potentially affecting actin-binding proteins like TDP-43 highlights its association with disease pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
See full target information EEF1A2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biology 14: PubMed41007382

2025

A Simple Three-Dimensional Compartmentalized Co-Culture Model for Basal Forebrain and Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Luo,Jing Li,Zhiyu Deng,Yali Xu,Xixi Li,Miao Ren,Xiangning Li
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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