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AB227737

Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

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Rabbit Recombinant Monoclonal EGFR deleted 746-750 + L747 + R748 + E749 + A750 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.
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Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)

Flow Cytometry analysis of HCC827(Human lung adenocarcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1 : 400 dilution (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)

Immunocytochemistry/ Immunofluorescence analysis of HCC827(human lung adenocarcinoma epithelial cell), negative control :
A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1 : 100 (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)
  • WB

Supplier Data

Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (AB227737)

All lanes:

Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737) at 1/300 dilution

All lanes:

HCC827 cell lysate

Predicted band size: 134 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Carrier free

    Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] - BSA and Azide free

  • 660 APC

    APC Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

  • 578 PE

    PE Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP111

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB226993

Anti-EGFR (phospho Y974) antibody

Lab Essentials

  • 0
  • 0
Primary Antibodies

AB227023

Anti-EGFR (phospho Y974) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y974) antibody (AB227023)

  • 0
  • 0
Primary Antibodies

AB81440

Anti-EGFR (phospho Y992) antibody [EM-12]

Lab Essentials

Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

  • 0
  • 0
Primary Antibodies

AB27764

PE Anti-EGFR antibody [ICR10]

Flow Cytometry - PE Anti-EGFR antibody [ICR10] (AB27764)

  • 0
  • 0
Primary Antibodies

AB231

Anti-EGFR antibody [ICR10]

20ul selling size|Lab Essentials|Recombinant

Flow Cytometry - Anti-EGFR antibody [ICR10] (AB231)

  • 4
  • 7
Primary Antibodies

AB11400

FITC Anti-EGFR antibody [ICR10]

Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (AB11400)

  • 4
  • 1
Primary Antibodies

AB194756

Anti-EGFR (phospho Y845) antibody

Lab Essentials

Western blot - Anti-EGFR (phospho Y845) antibody (AB194756)

  • 0
  • 0
Primary Antibodies

AB8465

Anti-EGFR antibody [2E9]

Lab Essentials

Flow Cytometry - Anti-EGFR antibody [2E9] (AB8465)

  • 4
  • 2
Primary Antibodies

AB131498

Anti-EGFR antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody (AB131498)

  • 0
  • 0
Primary Antibodies

AB5638

Anti-EGFR (phospho Y992) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y992) antibody (AB5638)

  • 0
  • 0
Primary Antibodies

AB5644

Anti-EGFR (phospho Y1068) antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody (AB5644)

  • 4
  • 5
Primary Antibodies

AB5651

Anti-EGFR (phospho Y1148) antibody

Lab Essentials

Immunocytochemistry - Anti-EGFR (phospho Y1148) antibody (AB5651)

  • 0
  • 0
Primary Antibodies

AB5652

Anti-EGFR (phospho Y1173) antibody

Lab Essentials

Immunocytochemistry - Anti-EGFR (phospho Y1173) antibody (AB5652)

  • 5
  • 1
Primary Antibodies

AB245363

Anti-EGFR antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody (AB245363)

  • 0
  • 0
Primary Antibodies

AB30

Anti-EGFR antibody [EGFR1]

BOND RX™ Validated|Lab Essentials|Trial Size

Flow Cytometry - Anti-EGFR antibody [EGFR1] (AB30)

  • 4
  • 4

보완 제품

Primary Antibodies

AB40815

Anti-EGFR (phospho Y1068) antibody [EP774Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)

  • 4
  • 14
Primary Antibodies

AB16661

Anti-Progesterone Receptor antibody [SP2]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

  • 4
  • 4
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p><p>Primary antibody incubation for 30 minutes at room temperature.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR del 746-750 + L747 + R748 + E749 + A750

대체 명칭 보기

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Bioengineered 13:5126-5140 PubMed35170372

2022

Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can Zou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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