Anti-EGFR (phospho Y1173) antibody

• ICC

Supplier Data

Immunocytochemistry - Anti-EGFR (phospho Y1173) antibody (AB5652)

HeLa cells stained for EGFR (green) using ab5652 at 2 μg/mL in ICC/IF. Followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization of EGFR (pY1173). Panel e shows untreated cells. Panel f shows no primary antibody control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody (AB5652)

Paraffin embedded human lung adenocarcinoma tissue stained for EGFR using ab5652 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody (AB5652)

Paraffin embedded human hepatocellular carcinoma tissue stained for EGFR using ab5652 at 1/20 dilution in immunohistochemical analysis.

• WB

Unknown

Western blot - Anti-EGFR (phospho Y1173) antibody (AB5652)

Cell extracts prepared from NIH3T3 cells expressing EGFR were starved for 30 hours, then stimulated for 10 minutes with 30 ng/mL EGF (+), or left unstimulated (-), then resolved by SDS-PAGE on a 6% Tris-glycine gel, and transferred to nitrocellulose. Membranes were incubated with 0.50 μg/mL ab5652 antibody, following prior incubation in the absence (lanes 1& 2), or presence of the peptide immunogen (lanes 3 & 4), or the nonphosphopeptide corresponding to the EGFR phosphopeptide (lanes 5 & 6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab5652 antibody for this phosphorylated residue. Cell extracts prepared from NIH3T3 cells expressing EGFR were starved for 30 hours, then stimulate

All lanes:

Western blot - Anti-EGFR (phospho Y1173) antibody (ab5652)

Predicted band size: 134 kDa

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