AB222888

Anti-EHD2 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal EHD2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EHD2 aa 400 to C-terminus.

대체 명칭 보기

PAST2, EHD2, EH domain-containing protein 2, PAST homolog 2

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EHD2 (green) using ab222888 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

Paraffin-embedded human kidney tissue stained for EHD2 using ab222888 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EHD2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZN4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EHD2 also known as EH domain-containing protein 2 is a mechanochemical protein with a molecular mass of approximately 61 kDa. It belongs to the dynamin superfamily of GTPases. EHD2 is frequently expressed in muscle cells adipocytes and cardiac tissues. It associates with cellular membranes particularly near caveolae where it plays a pivotal role in regulating cellular membrane dynamics and endocytosis through binding to phosphoinositides.

Biological function summary

The protein connection with membranes affects the regulation of vesicle trafficking and endosomal recycling. EHD2 functions as part of a complex with other proteins like pacsin and syndapin which modulates curvature of membranes a process essential for diverse cellular activities. By facilitating endocytosis EHD2 has a role in signaling pathways that influence cell migration and membrane repair.

Pathways

EHD2 interacts significantly with lipid raft-associated pathways which are critical in cellular communication and signal transduction. This protein interacts with the caveolin protein family particularly Caveolin-1 during caveolae-mediated endocytosis. It contributes to the spatial organization of signaling domains impacting the cellular response to external stimuli and stress.

The malfunction or altered expression of EHD2 associates with disorders such as obesity and muscle-related diseases. EHD2 dysregulation can affect Caveolin-1 contributing to abnormal vesicular transport and lipid metabolism. Such dysfunction holds potential links to the development or progression of conditions like cardiac hypertrophy highlighting the protein's importance in cellular health and disease.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed : 17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed : 25588833).

See full target information EHD2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Investigative ophthalmology & visual science 64:15 PubMed38095908

2023

JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Sun,Jiani Li,Jingyu Ma,Yuxing Zheng,Rong Ju,Xuri Li,Xiangrong Ren,Lijuan Huang,Rongyuan Chen,Xuhua Tan,Lixia Luo

International journal of clinical and experimental pathology 16:344-351 PubMed38059172

2023

EHD2, a novel HIF target gene, is a promising biomarker in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Chen,Song Xue,Jian Shi,Chunfeng He,Qingchuan Zhang

Nature communications 13:7234 PubMed36433988

2022

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Matthaeus,Kem A Sochacki,Andrea M Dickey,Dmytro Puchkov,Volker Haucke,Martin Lehmann,Justin W Taraska

Journal of thoracic disease 14:2652-2664 PubMed35928621

2022

inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Wei,Jingjing Shao,Jinming Wang,Tianye Zhao,Jia Liu,Xiying Shen,Yidan Wang,Haizhen Chen,Gaoren Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise