AB151694

Anti-EHD4 antibody [EPR9816(B)]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal EHD4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

HCA10, HCA11, PAST4, FKSG7, EHD4, EH domain-containing protein 4, Hepatocellular carcinoma-associated protein 10/11, PAST homolog 4

1 이미지

Unknown

Western blot - Anti-EHD4 antibody [EPR9816(B)] (AB151694)

All lanes:

Western blot - Anti-EHD4 antibody [EPR9816(B)] (ab151694) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9816(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with EHD3 and EHD1, 79% homology with EHD2. Cross-reactivity with this protein has not been confirmed experimentally.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EHD4 also known as EH domain-containing protein 4 is a member of the C-terminal Eps15 homology domain-containing protein family. This protein has a mass of approximately 68 kDa. Mechanically EHD4 participates in the regulation of endocytic transport by assisting in the remodeling of membranes. It is expressed in different tissues significantly in the brain and testes where it fulfills several cellular functions.

Biological function summary

EHD4 plays a role in intracellular trafficking processes essential for cellular homeostasis. It associates with the endosomal recycling complex facilitating the transport of receptors from endosomes back to the plasma membrane or the trans-Golgi network. This activity supports the maintenance of receptor availability on the cell surface influencing cellular responsiveness to external stimuli.

Pathways

EHD4 functions within endocytic and recycling pathways coordinating these with other cellular proteins. For instance EHD4 interacts with RAB proteins such as RAB11 to regulate membrane dynamics and vesicular traffic. These interactions are critical in the coordination and regulation of proteins involved in the recycling of membrane compartments within the cell.

EHD4 has been implicated in neurodegenerative diseases and certain forms of cancer. In the context of cancer EHD4's role in membrane trafficking affects cell signaling and proliferation while its dysregulation can lead to aberrant cellular behaviors. Furthermore it interacts with other proteins like EHD1 influencing pathophysiological processes linked to these conditions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport (PubMed : 17233914, PubMed : 18331452). During sprouting angiogenesis, in complex with PACSIN2 and MICALL1, forms recycling endosome-like tubular structure at asymmetric adherens junctions to control CDH5 trafficking (By similarity).

See full target information EHD4

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 11:2032-2043 PubMed32127931

2020

MRPS16 facilitates tumor progression via the PI3K/AKT/Snail signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wang,Junjun Li,Xiaobing Long,Liwu Jiao,Minghui Zhou,Kang Wu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHD4 antibody [EPR9816(B)]

Western blot - Anti-EHD4 antibody [EPR9816(B)] (AB151694)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise