Anti-eIF2A antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody (AB235147)

4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 μg/mL overnight at 4°C in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

• WB

Supplier Data

Western blot - Anti-eIF2A antibody (AB235147)

All lanes:

Western blot - Anti-eIF2A antibody (ab235147) at 5 µg/mL

Lane 1:

Daudi whole cell lysate at 35 µg

Lane 2:

MCF7 whole cell lysate at 35 µg

Lane 3:

HeLa whole cell lysate

Secondary

All lanes:

Donkey anti-Goat HRP at 1/40000 dilution

Predicted band size: 65 kDa

false

Exposure time: 35s

• WB

Supplier Data

Western blot - Anti-eIF2A antibody (AB235147)

All lanes:

Western blot - Anti-eIF2A antibody (ab235147) at 1/1000 dilution

Lane 1:

eIF2A human protein over HEK293 expressing lysate

Lane 2:

HEK293 lysate (empty vector)

Secondary

All lanes:

Donkey Fab2 anti-Goat HRP at 1/70000 dilution

Predicted band size: 65 kDa

false

Exposure time: 15s