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AB250752

Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal EIF2S2/EIF2B antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

EIF2B, EIF2S2, Eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit beta, eIF2-beta

4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)

This data was developed using ab184549, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2/EIF2B using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution, counterstained with Dapi.

Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)

This data was developed using ab184549, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed 293 cells labeling EIF2S2/EIF2B using ab184549 at a 1/160 dilution (red) or a Rabbit monoclonal IgG control (green).

Secondary antibody : Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)
  • IP

Supplier Data

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)

This data was developed using ab184549, the same antibody clone in a different buffer formulation.

Western blot analysis of EIF2S2/EIF2B in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.

All lanes:

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (<a href='/ko/products/primary-antibodies/eif2s2-eif2b-antibody-epr15833b-ab184549'>ab184549</a>)

Predicted band size: 38 kDa

false

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)
  • WB

Supplier Data

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free (AB250752)

This data was developed using ab184549, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (<a href='/ko/products/primary-antibodies/eif2s2-eif2b-antibody-epr15833b-ab184549'>ab184549</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15833(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB32157

Anti-EIF2S1 (phospho S51) antibody [E90]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

  • 4
  • 27
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB181186

Anti-EIF2B1 antibody [EPR13894(B)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] (AB181186)

  • 0
  • 0
Primary Antibodies

AB92809

Anti-CHREBP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHREBP antibody (AB92809)

  • 0
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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제품 세부 정보

ab250752 is the carrier-free version of ab184549.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2 and EIF2B part of eukaryotic translation initiation factor 2B are key players in the initiation of protein synthesis. EIF2S2 is a 38.3 kDa protein and is expressed in various tissues including the brain liver and muscle. These factors form a complex that facilitates the exchange of GDP for GTP on the EIF2 complex important for the delivery of initiator tRNA to the ribosome.
Biological function summary

EIF2S2 and EIF2B are part of the eukaryotic translation initiation factor complex which plays a significant role in protein synthesis regulation under stress conditions. This complex senses cellular stress and decreases global protein synthesis by controlling the phosphorylation status of EIF2 allowing the selective translation of stress response proteins. This mechanism helps cells to conserve resources during unfavorable conditions while still expressing proteins necessary for survival.

Pathways

EIF2S2/EIF2B orchestrates the integrated stress response (ISR) pathway and the unfolded protein response (UPR) pathway. These pathways sense disruptions in protein folding and energy stress respectively. The phosphorylation of EIF2α related to these pathways is an important regulatory step that reduces general protein synthesis and channels the cellular machinery towards resolving stress.

Defects in EIF2S2 and EIF2B contribute to disorders such as Vanishing White Matter disease and some cancers. Vanishing White Matter disease linked to mutations in EIF2B affects brain white matter and involves the regulation of stress responses through proteins like EIF2α. In certain cancers abnormalities in the EIF2 pathway can affect cell survival and proliferation linking the protein to tumorigenesis and cancer progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 31836389). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity).
See full target information EIF2S2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature cell biology 24:1541-1557 PubMed36192632

2022

Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana D Larionova,Soniya Bastola,Tatiana E Aksinina,Ksenia S Anufrieva,Jia Wang,Victoria O Shender,Dmitriy E Andreev,Tatiana F Kovalenko,Georgij P Arapidi,Polina V Shnaider,Anastasia N Kazakova,Yaroslav A Latyshev,Victor V Tatarskiy,Alexander A Shtil,Pascale Moreau,Francis Giraud,Chaoxi Li,Yichan Wang,Maria P Rubtsova,Olga A Dontsova,Michael Condro,Benjamin M Ellingson,Mikhail I Shakhparonov,Harley I Kornblum,Ichiro Nakano,Marat S Pavlyukov
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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