AB238921

Anti-eIF3B antibody [EPR5804] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal EIF3B antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

EIF3S9, EIF3B, Eukaryotic translation initiation factor 3 subunit B, eIF3b, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog, eIF-3-eta, eIF3 p110, eIF3 p116, hPrt1

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 μg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133601).

Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

Intracellular flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133601).

OI-RD Scanning - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5804

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab238921 is the carrier-free version of ab133601.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 3 subunit B (eIF3B) also known as EIF3S9 or p116 plays a central role in the initiation of translation. This protein has a mass of approximately 97 kDa and serves as an important component of the eIF3 complex important for assembling the 40S ribosomal subunit with mRNA and initiator tRNA. eIF3B is ubiquitously expressed in various tissues indicating its fundamental role in cellular protein synthesis.

Biological function summary

EIF3B functions as part of the large eIF3 complex which consists of 13 subunits. This complex is important for the recruitment of mRNA to the 40S ribosomal subunit playing an essential role in the start of translation. eIF3B directly contributes to stabilizing the interaction between mRNA and the ribosome promoting the accurate and efficient formation of the translation initiation complex. This function highlights the importance of eIF3B in regulating cellular protein levels.

Pathways

EIF3B participates in key processes of mRNA translation and is integral to the translation initiation pathway. It interacts with other translation initiation factors including eIF4F and eIF5 orchestrating the assembly and positioning of the ribosome for translation initiation. Moreover eIF3B is also involved in the mTOR signaling pathway linking nutrient sensing to protein synthesis regulation.

EIF3B has been associated with various types of cancer such as breast and prostate cancer where its increased expression correlates with tumor progression and poor prognosis. The dysfunction of eIF3B affects protein synthesis and cell proliferation contributing to oncogenesis. Furthermore eIF3B's role is connected to the dysregulation of mTOR pathways which is significant in cancer development. Its interaction with other proteins like eIF4E and eIF5 signifies its impact on cancer progression pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815, PubMed : 9388245). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632, PubMed : 9388245). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF3B antibody [EPR5804] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

OI-RD Scanning - Anti-eIF3B antibody [EPR5804] - BSA and Azide free (AB238921)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise