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AB134138

Anti-eIF4B (phospho S406) antibody [EPR7683]

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal eIF4B phospho S406 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

대체 명칭 보기

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)

Immunohistochemical analysis of Paraffin-embedded Human colon tissue labelling eIF4B phosphorylated at S406 with ab134138 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)
  • WB

Unknown

Western blot - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)

All lanes:

Western blot - Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) at 1/10000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Goat Ani-rabbit HRP at 1/2000 dilution

Predicted band size: 69 kDa

false

OI-RD Scanning - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-eIF4B (phospho S406) antibody [EPR7683] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7683

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB228800

Anti-eIF4B antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody - N-terminal (AB228800)

  • 0
  • 0
Primary Antibodies

AB59300

Anti-eIF4B (phospho S422) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S422) antibody (AB59300)

  • 0
  • 0
Primary Antibodies

AB129369

Anti-eIF4B antibody [1F5]

Flow Cytometry - Anti-eIF4B antibody [1F5] (AB129369)

  • 0
  • 0
Primary Antibodies

AB186856

Anti-eIF4B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody (AB186856)

  • 0
  • 0
Primary Antibodies

AB75823

Anti-eIF4B (phospho S504) antibody [EP2261Y]

RabMAb

Western blot - Anti-eIF4B (phospho S504) antibody [EP2261Y] (AB75823)

  • 0
  • 0
Primary Antibodies

AB245618

Anti-eIF4B antibody

Immunoprecipitation - Anti-eIF4B antibody (AB245618)

  • 0
  • 0
Primary Antibodies

AB68474

Anti-eIF4B antibody [EP2299Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

  • 5
  • 1

보완 제품

Primary Antibodies

AB188865

Anti-TXNIP antibody [EPR14774]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNIP antibody [EPR14774] (AB188865)

  • 4
  • 4
Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

  • 5
  • 4
Primary Antibodies

AB32024

Anti-eIF4EBP1 antibody [Y329]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y329] (AB32024)

  • 0
  • 0
Assay Kits

AB83463

Thioredoxin Reductase Assay Kit (Colorimetric)

Functional Studies - Thioredoxin Reductase Assay Kit (Colorimetric) (AB83463)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.
Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
See full target information EIF4B pS406
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cell and developmental biology 10:798316 PubMed35620059

2022

Single-Cell RNA Sequencing Profiles Identify Important Pathophysiologic Factors in the Progression of Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Lu,Li Li,Luolan Suo,Ping Huang,Hongjie Wang,Su Han,Mingming Cao

Cells 9: PubMed32883024

2020

Targeting Oxidative Phosphorylation Reverses Drug Resistance in Cancer Cells by Blocking Autophagy Recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Seon Lee,Ho Lee,Hyonchol Jang,Sang Myung Woo,Jong Bae Park,Seon-Hyeong Lee,Joon Hee Kang,Hee Yeon Kim,Jaewhan Song,Soo-Youl Kim

EBioMedicine 40:184-197 PubMed30686754

2019

Loss of SLC25A11 causes suppression of NSCLC and melanoma tumor formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Seon Lee,Ho Lee,Soohyun Lee,Joon Hee Kang,Seon-Hyeong Lee,Seul-Gi Kim,Eunae Sandra Cho,Nam Hee Kim,Jong In Yook,Soo-Youl Kim
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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