AB155482

Anti-eIF4GII antibody - N-terminal

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Rabbit Polyclonal eIF4GII antibody. Suitable for ICC/IF, WB, IHC-P, IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Eukaryotic translation initiation factor 4 gamma 3 aa 1-250.

대체 명칭 보기

Eukaryotic translation initiation factor 4 gamma 3, eIF-4-gamma 3, eIF-4G 3, eIF4G 3, eIF-4-gamma II, eIF4GII, EIF4G3

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-eIF4GII antibody - N-terminal (AB155482)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling EIF4G3 protein at cytoplasm using ab155482 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar= 10 µm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded rat hind brain tissue stained for EIF4G3 protein at cytosol with ab155482 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded mouse brain stem tissue stained for EIF4G3 protein at cytosol with ab155482 at a 1/500 dilution.

Antigen retrieval : EDTA based buffer, (pH 8.0), 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for EIF4G3 protein at cytoplasm with ab155482 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-eIF4GII antibody - N-terminal (AB155482)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-eIF4GII antibody - N-terminal (ab155482) at 1/1000 dilution

All lanes:

Mouse testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 177 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human Eukaryotic translation initiation factor 4 gamma 3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O43432

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 4GII (eIF4GII) also known as p97 plays an important role in the initiation of translation by acting as a central scaffold protein. With a molecular mass of approximately 160 kDa eIF4GII coordinates the assembly of the eIF4F translation initiation complex by interacting with other initiation factors and RNA. This protein is ubiquitously expressed across various tissues highlighting its fundamental role in protein synthesis.

Biological function summary

EIF4GII is integral to mRNA translation by facilitating the formation of the eIF4F complex which includes eIF4E eIF4A and itself. This complex binds to the 5' cap structure of mRNA an essential step for ribosome recruitment. eIF4GII performs functions important to translation regulation and cellular growth control influencing processes like cell proliferation and survival. Its activity links to mechanistic target mTOR signaling a pathway key to many cellular events.

Pathways

EIF4GII has significant involvement in the mTOR signaling pathway a major regulator of cell growth proliferation and protein synthesis. It operates alongside proteins such as eIF4E and eIF4A in this pathway facilitating the promotion of cap-dependent translation. eIF4GII also ties into the PI3K/AKT/mTOR axis influencing processes that control cell cycle progression and energy metabolism showing its role in the regulation of nutrient-sensitive signals.

EIF4GII has implications in cancer and neurodegenerative diseases linked through its control of protein synthesis. Aberrant expression or regulation of eIF4GII can lead to uncontrolled cell proliferation and survival common in various cancers. In these contexts proteins like mTOR and eIF4E interact with eIF4GII in oncogenic pathways. In neurodegenerative disorders altered translation initiation involving eIF4GII contributes to the pathogenesis impacting neuronal survival and function due to defective protein synthesis control.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed : 9418880). Functional homolog of EIF4G1 (PubMed : 9418880).

See full target information Eukaryotic translation initiation factor 4 gamma 3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Iranian journal of basic medical sciences 26:200-207 PubMed36742137

2023

ACTN1 interacts with ITGA5 to promote cell proliferation, invasion and epithelial-mesenchymal transformation in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Ying Gao,Huimin Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4GII antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4GII antibody - N-terminal (AB155482)

Western blot - Anti-eIF4GII antibody - N-terminal (AB155482)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise