JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB130402

Anti-ELOF1 antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal ELOF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ELOF1 aa 1 to C-terminus.

대체 명칭 보기

Transcription elongation factor 1 homolog, ELOF1

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ELOF1 antibody (AB130402)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ELOF1 antibody (AB130402)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab130402 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOF1 antibody (AB130402)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOF1 antibody (AB130402)

Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human lateral ventricle tissue labelled with ELOF1 antibody at 1/10 dilution.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ELOF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60002

style
left-column

보완 제품

Primary Antibodies

AB84340

Anti-Golgin 97 antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Golgin 97 antibody (AB84340)

  • 5
  • 6
Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB137072

Anti-PEX19 antibody [EPR9266(B)]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (AB137072)

  • 4
  • 1
Primary Antibodies

AB179503

Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)

  • 4
  • 3
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ELOF1 known as Elongation Factor Protein 1 plays a role in the transcription process. It assists RNA polymerase II with transcription elongation and maintaining transcript integrity. ELOF1 has a molecular weight of approximately 15 kDa. It is expressed in various tissues with higher levels found in active proliferating cells due to its role in gene expression regulation.
Biological function summary

ELOF1 supports the elongation phase of transcription and interacts with components of the transcription machinery. It forms part of a complex with other elongation factors helping RNA polymerase II proceed along DNA without stalling. ELOF1 ensures efficient synthesis of messenger RNA affecting the transcriptional output of numerous genes across different cellular processes.

Pathways

The influence of ELOF1 extends to key transcriptional and signaling pathways like the mRNA processing pathway. ELOF1 also connects closely to other transcription elongation factors like SPT6 affecting how cells respond to transcriptional cues. These connections are significant in maintaining proper gene regulation and expression in reaction to various intracellular signals.

ELOF1's interplay with transcription regulation links it to cancer and neurodegenerative diseases. Changes in ELOF1 expression or function can disrupt normal transcriptional elongation contributing to the uncontrolled growth of cancer cells. Additionally dysfunction in ELOF1 influences neurodegenerative processes likely through its regulatory effect on gene expression. It is associated with proteins like RNA polymerase II which play roles in these disease pathways.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Factor involved in transcription-coupled nucleotide excision repair (TC-NER), a mechanism that rapidly removes RNA polymerase II-blocking lesions from the transcribed strand of active genes (PubMed : 34108662, PubMed : 34108663, PubMed : 38316879). Acts as a key adapter required to anchor TC-NER factors to RNA polymerase II : stably positions UVSSA and the DCX(ERCC8) complex (also named CSA complex) on arrested RNA polymerase II, leading to neddylation and activation of the DCX(ERCC8) complex and ubiquitination of RNA polymerase II (PubMed : 38316879).
See full target information ELOF1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

OncoTargets and therapy 12:7437-7450 PubMed31686849

2019

Ultrasound-microbubbles-mediated microRNA-449a inhibits lung cancer cell growth via the regulation of Notch1.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwu Meng,Shaofei Yuan,Linjia Zhu,Zongxiao ShangGuan,Renguo Zhao
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com