AB94713

Anti-EMX2 antibody

Name: Anti-EMX2 antibody
Brand: Abcam Limited
SKU: AB94713
Rating: 5 (4 reviews)

(4 리뷰들)

(6 제품이 사용된 논문 )

Rabbit Polyclonal EMX2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 6 publications.

1 이미지

Lab

Western blot - Anti-EMX2 antibody (AB94713)

Blocking and diluting buffer and concentration : 1% NFDM/TBST.

Gel type : MES.

EMX2 contains both an O-linked glycosylation site and a phosphorylation site (UniProt) which may explain its migration at a higher molecular weight than predicted. Additional bands : 55 kDa (possible non-specific binding).

All lanes:

Western blot - Anti-EMX2 antibody (ab94713) at 1 µg/mL

Lane 1:

Human Brain lysate at 10 µg

Lane 2:

Mouse E10 Brain lysate at 10 µg

Lane 3:

Mouse E12 Brain lysate at 10 µg

Lane 4:

Mouse Brain lysate at 10 µg

Lane 5:

Mouse Hippocampus lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Exposure time: 20min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EMX2 also known as Empty Spiracles Homeobox 2 functions as a homeobox transcription factor. This protein has a mass of approximately 33 kilodaltons. It is mainly expressed in the developing brain where it plays an important role in brain morphogenesis and neuronal development. EMX2 also presents in other tissues such as the kidney and reproductive organs where it contributes to organogenesis.

Biological function summary

The EMX2 protein influences developmental processes by regulating gene expression essential for cellular differentiation and tissue development. It does not belong to a larger complex but interacts with various transcription factors to exert its regulatory functions. EMX2 modulates important aspects of brain development guiding the patterning and regional specification within the cerebral cortex.

Pathways

EMX2 plays an influential role in the Wnt signaling pathway which is key to developmental processes like cell fate determination proliferation and migration. Additionally EMX2 is involved in the regulation of neural lineage specification through its interaction with other proteins such as PAX6. Together these proteins create a regulatory network that controls neural precursor cell proliferation and differentiation.

EMX2 has implications in neurological disorders. Abnormal expression or mutations in the EMX2 gene can contribute to developmental brain disorders such as schizencephaly which involves abnormal clefts or deep grooves in the cerebral hemispheres. The protein also links to cancer pathways notably in gliomas where dysregulation of EMX2 expression can affect tumor progression. Connections between EMX2 and proteins like GLI1 in the Glioma signaling pathway provide insights into its role in oncogenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor, which in cooperation with EMX1, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combination with OTX1/2 to specify cell fates in the developing central nervous system. In the inner ear, it controls the distribution of GPR156 at hair cell boundaries, and regulates the organization of stereociliary bundles in opposite orientations across the line of polarity reversal (LPR).

See full target information EMX2

대체 명칭 보기

Homeobox protein EMX2, Empty spiracles homolog 2, Empty spiracles-like protein 2, EMX2

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록and refine your search

Nature protocols 20:1846-1883 PubMed39915620

2025

Generation of human fetal brain organoids and their CRISPR engineering for brain tumor modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Pagliaro,Francesco Andreatta,Roxy Finger,Benedetta Artegiani,Delilah Hendriks

Molecular carcinogenesis 63:951-961 PubMed38362840

2024

EMX2 inhibits clear cell renal cell carcinoma progress via modulating Akt/FOXO3a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Zhou,Sicheng Dong,Yuhao Zhou,Zhiqing He,Zhixiong Zhang,Liqiong Liao,Bangyu Zou,Xiaopeng Zheng,Kaoqing Peng,Xiaolu Duan

Molecular cancer 22:206 PubMed38093346

2023

Dynamic altruistic cooperation within breast tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Sufyan Bin Masroni,Kee Wah Lee,Victor Kwan Min Lee,Siok Bian Ng,Chao Teng Law,Kok Siong Poon,Bernett Teck-Kwong Lee,Zhehao Liu,Yuen Peng Tan,Wee Ling Chng,Steven Tucker,Lynette Su-Mien Ngo,George Wai Cheong Yip,Min En Nga,Susan Swee Shan Hue,Thomas Choudary Putti,Boon Huat Bay,Qingsong Lin,Lihan Zhou,Mikael Hartman,Tze Ping Loh,Manikandan Lakshmanan,Sook Yee Lee,Vinay Tergaonkar,Huiwen Chua,Adeline Voon Hui Lee,Eric Yew Meng Yeo,Mo-Huang Li,Chan Fong Chang,Zizheng Kee,Karen Mei-Ling Tan,Soo Yong Tan,Evelyn Siew-Chuan Koay,Marco Archetti,Sai Mun Leong

Frontiers in molecular neuroscience 15:865858 PubMed35875662

2022

Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene () and Its Molecular Target .

Applications

Unspecified application

Species

Unspecified reactive species

Karina D Rysenkova,Konstantin E Troyanovskiy,Polina S Klimovich,Taisiya R Bulyakova,Ekaterina M Shelomentseva,Anna A Shmakova,Daria Yu Tanygina,Olga I Ivashkina,Konstantin V Anokhin,Maxim N Karagyaur,Maria I Zvereva,Kseniya A Rubina,Vsevolod A Tkachuk,Ekaterina V Semina

Nature cell biology 24:981-995 PubMed35697781

2022

Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Rosebrock,Sneha Arora,Naresh Mutukula,Rotem Volkman,Elzbieta Gralinska,Anastasios Balaskas,Amèlia Aragonés Hernández,René Buschow,Björn Brändl,Franz-Josef Müller,Peter F Arndt,Martin Vingron,Yechiel Elkabetz

Journal of cellular biochemistry 120:6515-6527 PubMed30450775

2018

Wnt and BMP signaling pathways co-operatively induce the differentiation of multiple myeloma mesenchymal stem cells into osteoblasts by upregulating EMX2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fang Wei,Qiao-Lin Chen,Yuan Fu,Qi-Ke Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMX2 antibody

Western blot - Anti-EMX2 antibody (AB94713)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise