Anti-Endothelin 1 antibody [TR.ET.48.5]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

ab2786 labelling Endothelin 1 (green) in the secretion of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using ab2786.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

ab2786 labelling Endothelin 1 (green) in the secretion of HUVEC cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• Flow Cyt

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Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• Flow Cyt

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Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 293T cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• Flow Cyt

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Flow Cytometry - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

Flow cytometry analysis of Endothelin 1 showing positive staining in the cytoplasm of 3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ab2786 (0.5 ug/test) for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

ab2786 labelling Endothelin 1 (green) in the secretion of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• WB

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Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

All lanes:

Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) at 1/500 dilution

All lanes:

PC12 cell lysate at 25 µg

Predicted band size: 24 kDa

Observed band size: 30 kDa

false

• WB

CiteAb

Western blot - Anti-Endothelin 1 antibody [TR.ET.48.5] (AB2786)

Endothelin 1 western blot using anti-Endothelin 1 antibody [TR.ET.48.5] ab2786. Publication image and figure legend from Li, J. R., Zhao, Y. S., et al., 2018, PLoS One, PubMed 29641598.

ab2786 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab2786 please see the product overview.

Levels ET-1 in the serum and aorta when subjected to CIH.(A) ET-1 content was measured in serum from the Normoxia, CIH and CIH+Fa groups by radioimmunoassay. (B) ET-1 protein levels were measured in aortas isolated from the Normoxia, CIH and CIH + Fa groups by western blot. The results were expressed as the mean ± SE. *p < 0.05, CIH group vs Normoxia group; # p < 0.05, CIH + Fa group vs CIH group (n = 6 for each group).

false