AB222514

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ENO1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 2 publications.

대체 명칭 보기

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2, Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

10 이미지

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixedHeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ENO1 + ENO2 + ENO3with ab222514 at 1/800 (red) compared with an isotype control Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody Goat anti rabbit IgG Alexa Fluor^® 488 (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on medium-small ducts of human pancreas (PMID : 19425054). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded human clear cell carcinoma tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and nuclear staining on human clear cell kidney carcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

Counterstains : ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

Counterstains : ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded mouse pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on the medium-small ducts of normal mouse pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1-3 : 3 seconds

Lane 4 : 1 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1:

Human fetal heart at 10 µg

Lane 2:

Human fetal kidney at 10 µg

Lane 3:

Human fetal spleen at 10 µg

Lane 4:

Human fetal brain at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1/2/4 : 1 seconds

Lane 3 : 3 seconds

Lanes 1 - 3:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 4:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/10000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 3:

Human skeletal muscle at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lanes 1 & 2 : 3 seconds

Lane 3 : 1 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/1000 dilution

Lane 1:

His-tagged human ENO1 (aa2-434) recombinant protein, 10 ng

Lane 2:

His-tagged human ENO2 (aa1-434) recombinant protein, 10 ng

Lane 3:

His-tagged human ENO3 (aa1-434) recombinant protein, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1, 2 and 4 : 3 minutes

Lane 3 : 1 minute

Lane 5 : 3 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1:

Mouse brain at 10 µg

Lane 2:

Mouse spleen at 10 µg

Lane 3:

Mouse kidney at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19680

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/800", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ENO1 ENO2 and ENO3 also known as alpha gamma and beta enolase are isoenzymes involved in the glycolytic pathway. Each enolase isoenzyme offers specific mechanical functions in this energy-producing process. ENO1 has an approximate mass of 47 kDa and is widely expressed in most tissues. ENO2 similar in size is primarily found in neural and neuroendocrine tissues. ENO3 also roughly 47 kDa predominantly expresses in muscle tissue. These proteins catalyze the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate playing an important role in glycolysis.

Biological function summary

The enolase family of isoenzymes acts as multifunctional catalysts and has roles beyond glycolysis. ENO1 serves as a plasminogen receptor on cell surfaces assisting in tissue remodeling and immune response. ENO2 often referred to as neuron-specific enolase (NSE) functions importantly in neural development and tumor cell metabolism. ENO3 or muscle-specific enolase contributes to muscle metabolism and energy turnover. While these proteins individually carry out essential tasks they also form a complex that facilitates efficient energy production and cellular regulation.

Pathways

The enolase enzymes integrate in the glycolytic and gluconeogenesis pathways central to energy metabolism. Glycolysis involves other key proteins like hexokinase and phosphofructokinase cooperating for glucose breakdown and energy release. In gluconeogenesis the enolases help generate glucose from non-carbohydrate sources important during fasting or intense exercise. Their activity along with related proteins impacts the efficiency and regulation of energy metabolism pathways.

Enolase dysregulation associates with conditions like cancer and neuromuscular diseases. Elevated ENO2 levels often serve as a biomarker for neuroendocrine tumors and a target for cancer therapies. ENO3 mutations link to muscle metabolic disorders including glycogen storage diseases. ENO1 interaction with proteins such as plasminogen can influence cancer cell invasion and metastasis highlighting its relevance in disease progression and potential therapeutic interventions.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

Additional targets

ENO2,ENO3

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 15:1636367 PubMed40951359

2025

Claudin18.2 defines a prognostically distinct subgroup of intrahepatic cholangiocarcinoma via CD8 T-cell exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Chengui Yu,Yuren Pan,Fuli Li,Zhenyun Guo,Da Xu,Ying Zhu,Baobing Yin

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680]

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (2)

Product promise