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AB240352

Anti-eNOS antibody [EPR19296] - BSA and Azide free

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Rabbit Recombinant Monoclonal eNOS antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
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Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • IP

Lab

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

This data was developed using ab199956, the same antibody clone in a different buffer formulation.

eNOS was immunoprecipitated from HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 μg with ab199956 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab199956 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab199956 in HUVEC (human umbilical vein endothelial cell) whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 6s

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • IP

Supplier Data

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate 10 μg (Input).

Lane 2 : ab199956 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199956 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199956).

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>)

Predicted band size: 133 kDa

false

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • IP

Lab

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

This data was developed using ab199956, the same antibody clone in a different buffer formulation.

eNOS was immunoprecipitated from mouse kidney tissue lysate 10 μg with ab199956 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab199956 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab199956 in mouse kidney tissue lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

All lanes:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 41s

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • IP

Lab

Immunoprecipitation - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

This data was developed using ab199956, the same antibody clone in a different buffer formulation.

eNOS was immunoprecipitated from rat kidney tissue lysate 10 μg with ab199956 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab199956 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab199956 in rat kidney tissue lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

All lanes:

Rat kidney tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 76s

Western blot - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • WB

Lab

Western blot - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

This data was developed using ab199956, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

Lane 1:

Frozen HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Fresh HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 40s

Western blot - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)
  • WB

Unknown

Western blot - Anti-eNOS antibody [EPR19296] - BSA and Azide free (AB240352)

Exposure Time : Lane1 : 40 seconds; Lane2 : 180 seconds

The expression profile observed in HeLa is consistent with the literature (PMID : 19925457).
Negative control : HeLa (PMID : 19925457)

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] - BSA and Azide free (ab240352) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19296

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB76198

Anti-eNOS antibody [M221]

BOND RX™ Validated

Western blot - Anti-eNOS antibody [M221] (AB76198)

  • 5
  • 15
Primary Antibodies

AB5589

Anti-eNOS antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (AB5589)

  • 4
  • 8
Primary Antibodies

AB230455

Anti-eNOS antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody - C-terminal (AB230455)

  • 0
  • 0
Primary Antibodies

AB215717

Anti-eNOS (phospho S1177) antibody [EPR20991]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)

  • 0
  • 0
Primary Antibodies

AB230158

Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

  • 0
  • 0
Primary Antibodies

AB184154

Anti-eNOS (phospho S1177) antibody

  • 4
  • 2

보완 제품

Assay Kits

AB65328

Nitric Oxide Assay Kit (Colorimetric)

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

  • 4
  • 3
Primary Antibodies

AB215717

Anti-eNOS (phospho S1177) antibody [EPR20991]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 17
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.</p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.</p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.</p>" } } }
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제품 세부 정보

ab240352 is the carrier-free version of ab199956.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
See full target information NOS3

대체 명칭 보기

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental dermatology 33:e15009 PubMed38284185

2024

Road-traffic-related air pollution contributes to skin barrier alteration and growth defect of sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Christelle Le Gall-Lanotto,Anthony Verdin,Fabrice Cazier,Adeline Bataille-Savattier,Christelle Guéré,Marie M Dorr,Joachim W Fluhr,Dominique Courcot,Katell Vié,Laurent Misery
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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