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AB242741

Anti-eNOS antibody [EPR19332-22] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal eNOS antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3

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Sandwich ELISA - Anti-eNOS antibody [EPR19332-22] - BSA and Azide free (Capture) (AB242741)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-eNOS antibody [EPR19332-22] - BSA and Azide free (Capture) (AB242741)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab263878).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19332-22

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB65328

Nitric Oxide Assay Kit (Colorimetric)

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

  • 4
  • 3
Primary Antibodies

AB215717

Anti-eNOS (phospho S1177) antibody [EPR20991]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)

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  • 0
Primary Antibodies

AB269380

Anti-Lysozyme antibody [EPR22270-53] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Lysozyme antibody [EPR22270-53] - BSA and Azide free (Capture) (AB269380)

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  • 0
Primary Antibodies

AB285085

Anti-Kisspeptin antibody [EPR23770-178] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Kisspeptin antibody [EPR23770-178] - BSA and Azide free (Detector) (AB285085)

  • 0
  • 0
Primary Antibodies

AB254348

Anti-160 kD Neurofilament Medium antibody [EPR23510-76] - Neuronal Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [EPR23510-76] - Neuronal Marker (AB254348)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242741 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human eNOS. The recommended pair for sandwich ELISA is:
Capture: ab242741, Human eNOS Capture Antibody (unconjugated)
Detector: ab242933, Human eNOS Detector Antibody (unconjugated)
The reference range value is 234.38 - 15000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
See full target information NOS3
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Product promise

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