Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Overlay histogram showing MCF-7 (Human breast adenocarcinoma) cells stained with ab216136 (blue) or isotype control (red). The cells were fixed with paraformaldehyde and incubated with ab216136 at 1 ug/million cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Immunohistochemical analysis of paraffin embedded, formalin fixed human breast carcinoma tissue labeling EpCAM with ab216136 at 1 μg/mL.

• WB

Lab

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Lanes 1 - 4 : Merged signal (red and green). Green - ab216136 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab216136 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EPCAM knockout sample. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab216136 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) at 0.5 µg/mL

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

EPCAM knockout A431 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 35 kDa

Observed band size: 40 kDa

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