AB46714

Anti-EpCAM antibody [HEA125], prediluted

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(4 제품이 사용된 논문 )

Mouse Monoclonal EPCAM antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)

ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HEA125

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

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대체품

Primary Antibodies

AB237387

PE Anti-EpCAM antibody [EPR20533-63]

RabMAb|Recombinant

Flow Cytometry - PE Anti-EpCAM antibody [EPR20533-63] (AB237387)

Primary Antibodies

AB213500

Anti-EpCAM antibody [EPR20532-222]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-222] (AB213500)

Primary Antibodies

AB124825

Anti-EpCAM antibody [EPR677(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Primary Antibodies

AB225894

Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (AB225894)

Primary Antibodies

AB221552

Anti-EpCAM antibody [EPR20533-63]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] (AB221552)

Primary Antibodies

AB213501

Anti-EpCAM antibody [EPR20533-266]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] (AB213501)

Primary Antibodies

AB32392

Anti-EpCAM antibody [E144]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-EpCAM antibody [E144] (AB32392)

Primary Antibodies

AB223582

Anti-EpCAM antibody [EPR20532-225]

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] (AB223582)

보완 제품

Assay Kits

AB264632

Human EpCAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EpCAM ELISA Kit (AB264632)

Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

Primary Antibodies

AB307134

Alexa Fluor® 488 Anti-CD3 antibody [SP162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD3 antibody [SP162] (AB307134)

Primary Antibodies

AB155382

APC Anti-CCR7 antibody [3D12]

Flow Cytometry - APC Anti-CCR7 antibody [3D12] (AB155382)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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제품 세부 정보

ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: BSA, PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

See full target information EPCAM

대체 명칭 보기

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ImmunoHorizons 8:500-510 PubMed39018546

2024

Comprehensive Flow Cytometric, Immunohistologic, and Molecular Assessment of Thymus Function in Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Laura P Hale,Andrew N Macintyre,Dawn E Bowles,Jean Kwun,Jie Li,Barbara Theriot,Joseph W Turek

Bio-protocol 8:e3124 PubMed34532562

2018

Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Vikash Kumar,Asok Mukhopadhyay

Nature communications 9:894 PubMed29497050

2018

Whole-exome sequencing reveals the origin and evolution of hepato-cholangiocarcinoma.

Applications

IHC-P

Species

Human

Anqiang Wang,Liangcai Wu,Jianzhen Lin,Longzhe Han,Jin Bian,Yan Wu,Simon C Robson,Lai Xue,Yunxia Ge,Xinting Sang,Wenze Wang,Haitao Zhao

World journal of hepatology 5:425-32 PubMed24023981

2013

Participation of peribiliary glands in biliary tract pathophysiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Saya Igarashi,Yasunori Sato,Xiang Shan Ren,Kenichi Harada,Motoko Sasaki,Yasuni Nakanuma

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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