AB300524

Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal EPCR/CD201 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples.

대체 명칭 보기

CD201, Epcr, Procr, Endothelial protein C receptor, Activated protein C receptor, Centrocyclin, Centrosomal protein CCD41, Endothelial cell protein C receptor, APC receptor

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

This data was developed using ab300523, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelioma endothelialpolyoma middle T antigen transformed) cells labelling EPCR/CD201 with ab300523 at 1/500 (0.988 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in bEnd.3 cell line low expression : B16-F10 (PMID : 19188668) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

Flow cytometric analysis of B16-F10 (Mouse skin melanoma, Left) / bEnd.3 (mouse brain endothelioma, Right) cells labelling EPCR/CD201 with ab300523 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Low expression : B16-F10 (PMID : 19188668).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26267-19

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EPCR also known as CD201 stands for Endothelial Protein C Receptor. It is a glycoprotein with a molecular mass of approximately 46 kDa. EPCR is expressed mainly on endothelial cells which line the interior surface of blood vessels. This receptor plays a mechanical role in binding to protein C a zymogen that is converted to activated protein C (APC) by thrombin-thrombomodulin complex. By facilitating the activation of protein C EPCR contributes to the regulation of blood coagulation and inflammation processes.

Biological function summary

The EPCR is part of the protein C anticoagulant pathway where it acts as a cofactor. EPCR is vital in enhancing the activation of protein C on the endothelial surface reducing coagulation activity. It interacts closely with thrombomodulin and protein C to form this complex preventing excessive thrombus formation and contributing to vascular integrity. Apart from its anticoagulant role EPCR also modulates inflammatory responses supporting its function as a bridge between coagulation and inflammation.

Pathways

EPCR lies within the protein C anticoagulation pathway and the endothelial signaling pathways. Its primary partner in the anticoagulation pathway is APC which degrades activated factors Va and VIIIa providing a check against coagulation. Additionally EPCR’s interaction with APC and thrombomodulin links this protein to the endothelial signaling pathway where it affects cell survival and inflammation regulation. This interaction is essential to maintain hemostatic balance and control vascular responses to inflammatory stimuli.

EPCR associates with conditions like thrombosis and sepsis. In thrombosis inadequate expression or function of EPCR can lead to uncontrolled blood clotting due to insufficient activation of protein C. Sepsis a severe inflammatory response to infection shows abnormal modulation of the protein C pathway where EPCR's role becomes pivotal. Additionally APC which interacts directly with EPCR is critical in the pathological states of these diseases highlighting the therapeutic potential in targeting EPCR pathway.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

See full target information Procr

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free)

Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

Flow Cytometry (Intracellular) - Anti-EPCR/CD201 antibody [EPR26267-19] (BSA and Azide free) (AB300524)

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise