AB193634

Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal Ephrin B1 phospho Y324 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EFNB2 pY311.

대체 명칭 보기

EFL3, EPLG2, LERK2, EFNB1, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, ELK-L, LERK-2, EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L, EPLG8, LERK8, EFNB3, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) with ab193634 at a 1/50 dilution. Right image is treated with blocking peptide.

Supplier Data

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

All lanes:

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells treated with serum

Lane 2:

Extracts from HepG2 cells treated with serum with antigen-specific peptide

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human EFNB1 pY324 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database link P98172

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human EFNB3 pY318. Database link Q15768

Specificity

ab193634 detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318 respectivly.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab193634 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin B1 Ephrin B2 and Ephrin B3 also known as EFNB1 EFNB2 and EFNB3 are transmembrane proteins involved in cell communication. These proteins belong to the ephrin family and typically exhibit molecular masses ranging from 25 to 30 kDa. They are predominantly expressed in the nervous system vasculature and various tissues such as bones and liver. By binding to Eph receptors which are a subclass of receptor tyrosine kinases ephrins play a major role in bidirectional signaling between adjacent cells.

Biological function summary

These ephrins operate together with Eph receptors in a larger signaling complex. They influence cellular processes like boundary formation neural development and angiogenesis. Their interaction dictates the positioning and migration of cells during development. Ephrin B2 and Ephrin B3 specifically guide the formation of neural pathways and support vascularization while Ephrin B1 is important for craniofacial development. The dynamic interactions within these complexes highlight their regulatory role across various stages of organismal development.

Pathways

These ephrins and Eph receptors are integral to the Eph/ephrin signaling pathway and the MAPK signaling pathway. The Eph/ephrin pathway governs cellular repulsion and attraction mechanisms critical for tissue patterning. Ephrin B2 and Ephrin B3 through their interaction with EphB receptors influence these signaling cascades. In the MAPK pathway their activity affects cell proliferation and differentiation by interacting with proteins like RAS and RAF which contribute to signaling cascades that impact cell fate decisions.

These ephrins are associated with developmental defects and cancer. Mutations or dysregulation in Ephrin B1 can lead to craniofrontonasal syndrome characterized by abnormal facial and skeletal development. Ephrin B2 and Ephrin B3 have connections to tumor growth and metastasis often associated with aberrant angiogenesis. Interplay with proteins like VEGF in tumor environments highlights their role in promoting aberrant vascular networks contributing to cancer progression.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed : 7973638, PubMed : 8070404). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed : 7973638, PubMed : 8070404). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed : 7973638, PubMed : 8070404). Can also bind EPHB2 and EPHB3 (PubMed : 8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

See full target information EFNB1 pY324

Additional targets

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cellular and molecular life sciences : CMLS 79:487 PubMed35984507

2022

WWOX inhibition by Zfra1-31 restores mitochondrial homeostasis and viability of neuronal cells exposed to high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Carvalho,Sónia C Correia,Raquel Seiça,Paula I Moreira

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (1)

Product promise