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AB272359

Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector)

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Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector) (ab272359) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting EPO in ELISA. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

Erythropoietin, Epo, EP, EPO alpha, Epoetin, Erythropoetin, Erythropoietin precursor, MVCD2

1 이미지
Sandwich ELISA - Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector) (AB272359)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-EPO antibody [EPR22931-29] - BSA and Azide free (Detector) (AB272359)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab274397).

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Biotin

    Biotin Anti-EPO antibody [EPR22931-29] (Detector)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22931-29

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB234046

Hemoglobin Assay Kit (Colorimetric)

Functional Studies - Hemoglobin Assay Kit (Colorimetric) (AB234046)

  • 0
  • 0
Assay Kits

AB253419

EPO Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - EPO Antibody Pair - BSA and Azide free (AB253419)

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Primary Antibodies

AB267450

Anti-Visfatin antibody [EPR21980-17] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Visfatin antibody [EPR21980-17] - BSA and Azide free (Detector) (AB267450)

  • 0
  • 0
Primary Antibodies

AB242692

Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) (AB242692)

  • 0
  • 0
Primary Antibodies

AB244908

Anti-Thrombopoietin antibody [EPR14948-169] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Thrombopoietin antibody [EPR14948-169] - BSA and Azide free (Detector) (AB244908)

  • 0
  • 0
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Reactivity 정보

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제품 세부 정보

ab272359 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat Erythropoietin. The recommended pair for sandwich ELISA is:
Capture: ab272358, Human/Mouse/Rat Erythropoietin Capture Antibody (unconjugated)
Detector: ab272359, Human/Mouse/Rat Erythropoietin Detector Antibody (unconjugated)
The reference range value is the reference range value is 3.125 - 200 pg/ml for human, 9.375 - 600 pg/ml for mouse, and 11.719 - 750 pg/ml for rat.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.
Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
See full target information Epo

Additional targets

EPO
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Product promise

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