AB185699

Anti-EPPIN antibody

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Rabbit Polyclonal EPPIN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPPIN.

대체 명칭 보기

SPINLW1, WAP7, WFDC7, EPPIN, Eppin, Cancer/testis antigen 71, Epididymal protease inhibitor, Protease inhibitor WAP7, Serine protease inhibitor-like with Kunitz and WAP domains 1, WAP four-disulfide core domain protein 7, CT71

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-EPPIN antibody (AB185699)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPPIN antibody (AB185699)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab185699. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPIN antibody (AB185699)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPIN antibody (AB185699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling EPPIN with ab185699 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-EPPIN antibody (AB185699)

All lanes:

Western blot - Anti-EPPIN antibody (ab185699) at 1/500 dilution

Lane 1:

THP1 cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

Mouse testis lysate

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EPPIN.

O95925

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EPPIN short for Epididymal protease inhibitor is a protein that plays an important structural and mechanical role in sperm functionality. With a molecular mass of approximately 19 kDa EPPIN is mainly expressed in the male reproductive system particularly in the epididymis and on the surface of spermatozoa. This protein is recognized by several names including SPINLW1 which stands for Sperm protein with a Kunitz-type inhibitor domain. EPPIN interacts with the surface of sperm to regulate sperm motility and maturation.

Biological function summary

EPPIN influences sperm function by acting as part of a multi-protein complex essential for sperm motility and stability. It is known to bind with Semenogelin (SEMG) a major protein in semen impacting its hydrolytic and inhibitory action. Through this interaction EPPIN contributes to the liquefaction of semen and the regulation of sperm forward motility. It modulates calcium signaling in spermatozoa which is critical for the regulation of sperm activity post-ejaculation.

Pathways

EPPIN has been implicated in pathways associated with reproductive processes. It plays a role in the regulation of the calcium signaling pathway which involves other proteins like CatSper which are essential for sperm capacitation and hyperactivation. EPPIN through its interaction with SEMG indirectly influences the proteolytic pathways that govern seminal fluid conversion from gel to liquid state necessary for the sperm to become fully motile.

EPPIN has connections with male infertility and potential as a target for male contraception. Its interaction with SEMG has been studied in relation to infertility cases where sperm motility is compromised. Furthermore altered levels or functions of EPPIN could link to abnormal semen viscosity contributing to difficulties in natural conception. Researchers are exploring EPPIN as a target for non-hormonal male contraceptives leveraging its pivotal role in sperm functionality without affecting hormone levels.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility.

See full target information EPPIN

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70090 PubMed39432244

2024

METTL3-mediated m6A modification enhances lncRNA H19 stability to promote endothelial cell inflammation and pyroptosis to aggravate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Tang,Long-Hai Tian,Xiao-Han Zhu,Sen Yang,Huan Zeng,Yong-Yao Yang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPPIN antibody

Immunocytochemistry/ Immunofluorescence - Anti-EPPIN antibody (AB185699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPIN antibody (AB185699)

Western blot - Anti-EPPIN antibody (AB185699)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise