AB242195

Anti-ErbB2 / HER2 antibody [CL0268]

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Mouse Monoclonal ErbB2 / HER2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ERBB2 aa 250 to C-terminus.

대체 명칭 보기

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (AB242195)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (AB242195)

Paraffin-embedded human HER2-positive breast cancer tissue stained for ErbB 2 using ab242195 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (AB242195)

Negative control :

Paraffin-embedded human HER2-negative breast cancer tissue stained for ErbB 2 using ab242195 at 1/50 dilution in immunohistochemical analysis. No positive ErbB 2 staining as expected.

Supplier Data

Western blot - Anti-ErbB2 / HER2 antibody [CL0268] (AB242195)

All lanes:

Western blot - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195) at 1 µg/mL

Lane 1:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate.

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 137 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0268

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ERBB2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04626

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대체품

Primary Antibodies

AB214275

Anti-ErbB2 / HER2 antibody [EPR19547-12]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275)

Primary Antibodies

AB134182

Anti-ErbB2 / HER2 antibody [EP1045Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (AB134182)

Primary Antibodies

AB108371

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

Primary Antibodies

AB53290

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (AB53290)

Primary Antibodies

AB194979

Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979)

Primary Antibodies

AB225509

Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EPR19547-12]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB225509)

Primary Antibodies

AB281782

Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y] (AB281782)

Primary Antibodies

AB225510

Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EPR19547-12]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB225510)

보완 제품

Assay Kits

AB283881

Human ErbB2 / HER2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

Primary Antibodies

AB52894

Anti-EGFR antibody [EP38Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (AB52894)

Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

See full target information ERBB2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Development (Cambridge, England) 148: PubMed34184026

2021

scRNA sequencing uncovers a TCF4-dependent transcription factor network regulating commissure development in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Theres Wittmann,Sayako Katada,Elisabeth Sock,Philipp Kirchner,Arif B Ekici,Michael Wegner,Kinichi Nakashima,Dieter Chichung Lie,André Reis

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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