Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

This data was developed using ab231438, the same antibody clone in a different buffer formulation. Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using ab231438 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling ErbB2 / HER2 with ab231438 at 1/100 dilution (6.38 μg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10mins Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab231438 for 30 mins at room temperature. This image was generated using ab231438, the same clone, but with a different buffer formulation.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

This data was developed using ab231438, the same antibody clone in a different buffer formulation. Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling ErbB2 / HER2 with ab231438 at 1/100 dilution (1.59 µg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland. Panel B : anti-PR stained on nucleus of some ductal cells. Panel C : anti-HER2 stained on no cells. Panel D : anti-ER stained on nucleus of some ductal cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

This data was developed using ab231438, the same antibody clone in a different buffer formulation. Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling ErbB2 / HER2 with ab231438 at 1/100 dilution (1.59 µg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma. Panel B : anti-PR stained on no cells. Panel C : anti-HER2 stained on no cells. Panel D : anti-ER stained on no cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

This data was developed using ab231438, the same antibody clone in a different buffer formulation. Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling ErbB2 / HER2 with ab231438 at 1/100 dilution (1.59 µg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain. Panel A : merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma. Panel B : anti-PR stained on nucleus of cancer cells. Panel C : anti-HER2 stained on membrane of cancer cells. Panel D : anti-ER stained on nucleus of cancer cells. The section was incubated in three rounds of staining : in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• WB

Lab

Western blot - Anti-ErbB2 / HER2 antibody [SP101] - BSA and Azide free (AB240410)

This data was developed using ab231438, the same antibody clone in a different buffer formulation.

Blocking/Diluting Buffer and concentration : 5% NFDM /TBST

ab231438 concentration is assay dependent.

All lanes:

Western blot - Anti-ErbB2 / HER2 antibody [SP101] (<a href='/ko/products/primary-antibodies/erbb2-her2-antibody-sp101-ab231438'>ab231438</a>)

All lanes:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 137 kDa

Observed band size: 180 kDa

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