JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB16662

Anti-ErbB2 / HER2 antibody [SP3]

Anti-ErbB2 / HER2 항체 [SP3]

5

(2 리뷰들)

|

(10 제품이 사용된 논문 )

Anti-ErbB2 / HER2 antibody [SP3] (ab16662) is a rabbit monoclonal antibody detecting ErbB2 / HER2 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2005
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)

Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling ErbB2 with ab16662 at a concentration of 0.16 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab16662 anti-ErbB2 / HER2 antibody [SP3] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)

Formalin-fixed, paraffin-embedded human breast invasive ductal carcinoma tissue stained for ErbB2 / HER2 using ab16662 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG HRP. Heat mediated antigen retrieval was done using a pH 8.5 buffer.

This image was generated from the hybridoma version.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (AB16662)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab16662, followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on Human breast carcinoma, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab16662 for 10 mins at room temperature. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10 minutes. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

This image was generated from the hybridoma version.

관련 conjugated 항체와 다양한 조성의 항체 (4)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP3

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Extracellular domain

Specificity

<p>This antibody has some non-specific staining signals in skeletal muscle tissues in IHC-P. For better sensitivity and less background, we recommend ab134182 and ab237715 as alternatives.</p>

style
left-column

대체품

Primary Antibodies

AB214275

Anti-ErbB2 / HER2 antibody [EPR19547-12]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB214275)

  • 5
  • 3
Primary Antibodies

AB134182

Anti-ErbB2 / HER2 antibody [EP1045Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (AB134182)

  • 4
  • 4
Primary Antibodies

AB108371

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (AB108371)

  • 2
  • 1
Primary Antibodies

AB53290

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (AB53290)

  • 5
  • 1
Primary Antibodies

AB194979

Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (AB194979)

  • 0
  • 0
Primary Antibodies

AB225509

Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EPR19547-12]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB225509)

  • 5
  • 1
Primary Antibodies

AB281782

Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-ErbB2 / HER2 antibody [EP1045Y] (AB281782)

  • 0
  • 0
Primary Antibodies

AB225510

Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EPR19547-12]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EPR19547-12] (AB225510)

  • 0
  • 0

보완 제품

Assay Kits

AB283881

Human ErbB2 / HER2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB16660

Anti-Estrogen Receptor alpha antibody [SP1]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (AB16660)

  • 4
  • 7
Primary Antibodies

AB16661

Anti-Progesterone Receptor antibody [SP2]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

  • 4
  • 4
Primary Antibodies

AB52894

Anti-EGFR antibody [EP38Y]

BOND RX™ Validated|KO Validated|RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (AB52894)

  • 5
  • 24
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>ERBB2 is expressed at very low or undetectable levels in normal human breast tissue, and only a subset of breast cancer cases are ERBB2-positive.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column

제품 세부 정보

This product was switched from a hybridoma to recombinant production method on 5th March 2019.

Product Specifications
Anti-ErbB2 / HER2 antibody [SP3] (ab16662) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P in human samples.
Anti-ErbB2 / HER2 antibody [SP3] (ab16662) specifically detects ErbB2 / HER2 (UniProt ID: P04626; Molecular weight: 136kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Anti-ErbB2 / HER2 antibody [SP3] (ab16662) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-ErbB2 / HER2 antibody [SP3] (ab16662) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Related Products
Antibody clone SP3 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (ab270190, ab270191).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
See full target information ERBB2

대체 명칭 보기

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19
style
left-column

제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 28:451 PubMed39100992

2024

Newer combination treatments for breast cancer coexisting with acute myeloid leukemia in the novel regimens era: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Xing,Tian Tian,Yang Li,Jingnan Zhang,Xiaonan Guo,Shukai Qiao

Journal of clinical medicine 11: PubMed36498537

2022

HER2 Expression in Peritoneal Dissemination of High-Grade Serous Ovarian Carcinoma: A Comparative Study of Immunohistochemical Reactivity Using Four HER2 Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Kyung Yeo,Sup Kim,Heon Jong Yoo,Kwang-Sun Suh,Kyung-Hee Kim

Journal of oncology 2022:9390912 PubMed36046364

2022

Combining Organoid Models with Next-Generation Sequencing to Reveal Tumor Heterogeneity and Predict Therapeutic Response in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhong Liu,Yixiang Gan,NiJiati AiErken,Wei Chen,Shiwei Zhang,Jie Ouyang,Leli Zeng,Di Tang

Scientific reports 12:12894 PubMed35902718

2022

EGFR, HER2, and HER3 protein expression in paired primary tumor and lymph node metastasis of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Ye,Fanghua Li,Yuanyuan Wei,Yihao Zhang,Jianing Cui,Rui Dai,Hao Chen,Jing Xie,Peiling Cai

International journal of molecular sciences 23: PubMed35269608

2022

In Vivo Evaluation of Sgc8-c Aptamer as a Molecular Imaging Probe for Colon Cancer in a Mouse Xenograft Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Paula Arévalo,Romina Castelli,Manuel Ibarra,Martina Crispo,Victoria Calzada

Oncology letters 20:129 PubMed32934698

2020

Genetic profiling of somatic alterations by Oncomine Focus Assay in Korean patients with advanced gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joonhong Park,Sang-Il Lee,Soyoung Shin,Jang Hee Hong,Han Mo Yoo,Jeong Goo Kim

Cell proliferation 52:e12612 PubMed31012189

2019

ERα is required for suppressing OCT4-induced proliferation of breast cancer cells via DNMT1/ISL1/ERK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Jin,Yanru Li,Yantong Guo,Yiyang Jia,Huinan Qu,Yan Lu,Peiye Song,Xiaoli Zhang,Yijia Shao,Da Qi,Wenhong Xu,Chengshi Quan

Journal of cancer metastasis and treatment 3:150-160 PubMed28955730

2017

TGF-β Stimulation of EMT Programs Elicits Non-genomic ER-α Activity and Anti-estrogen Resistance in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maozhen Tian,William P Schiemann

Asian Pacific journal of cancer prevention : APJCP 15:9739-45 PubMed25520097

2014

Hormone receptor, HER2/NEU and EGFR expression in ovarian carcinoma--is here a prognostic phenotype?

Applications

Unspecified application

Species

Unspecified reactive species

Lutfiye Demir,Seyran Yigit,Canan Sadullahoglu,Murat Akyol,Suna Cokmert,Yuksel Kucukzeybek,Ahmet Alacacioglu,Fulya Cakalagaoglu,Mustafa Oktay Tarhan

The British journal of radiology 88:20140638 PubMed25429418

2014

Does background parenchymal enhancement on MRI affect the rate of positive resection margin in breast cancer patients?

Applications

Unspecified application

Species

Unspecified reactive species

S Y Park,D K Kang,T H Kim
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com