AB326662

Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274]

Recombinant
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Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1139 antibody. Suitable for WB and reacts with Human samples.

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Supplier Data

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : A431 cell lysate
Lane 2 : A431 treated with 100ng/ml EGF 10min
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 138 kDa
Observed band size : 180 kDa
Exposure time : 13 seconds

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (ab326662) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

A431 treated with 100ng/ml EGF 10min at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 180 kDa

false

Exposure time: 13s

Supplier Data

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : ErbB2 (Phospho-Tyr1139) knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (ab326662) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ErbB2 / HER2 (phospho Y1139) knockout A549 cell lysate at 20 µg

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1274

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

See full target information ERBB2 pY1139

대체 명칭 보기

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274]

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [SR1274] (AB326662)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise